Ibicuruzwa

Tricine, ni zwitterionic buffer reagent izina ryayo rikomoka kuri Tris na glycine.Imiterere yacyo isa na Tris, ariko kwibanda kwayo bifite ibikorwa byo kubuza imbaraga kurusha Tris.Imwe muma buffer ya reagent, yambere yatunganijwe kugirango itange sisitemu ya chloroplast reaction.Urutonde rwiza rwa pH rwa Tricine ni 7.4-8.8, pKa = 8.1 (25 ° C), kandi rusanzwe rukoreshwa nka buffer ikora no guhagarika pellet selile.Tricine ifite ibiranga umuriro muke hamwe nimbaraga za ionic nyinshi, zikwiranye cyane no gutandukanya electrophoreque yo gutandukanya poroteyine zifite uburemere buke bwa 1 ~ 100 kDa.Muri firefly luciferase ishingiye kuri ATP, ugereranije na buffer 10 zisanzwe, Tricine (25 mM) yerekanye ingaruka nziza zo gutahura.Byongeye kandi, Tricine nubundi buryo bwiza bwa hydroxyl radical scavenger mubushakashatsi bwangiza bwa radical yubusa.