Ibicuruzwa

Iriburiro:Tris-Hydroxymethylaminomethane hydrochloride yitwa Tris-HCl.Buffer ya Tris-HCl (buffer ya Tris-HCl) ntabwo ikoreshwa cyane nk'umuti wa acide nucleique na proteyine, ariko kandi ifite akamaro gakomeye.

Gusaba:Tris-HCl yakoreshejwe mu gukura kwa poroteyine ya kirisiti mu bihe bitandukanye bya pH.Imbaraga nkeya ionic ya buffer ya Tris-HCL irashobora gukoreshwa mugukora fibre intera intera ya lamin muri C. elegans.Tris-HCl nayo ni kimwe mu bintu by'ingenzi bigize poroteyine electrophorei.Byongeye kandi, Tris-HCL nayo ni intera yo gutegura surfactants, yihuta y’ibirunga hamwe n’ibiyobyabwenge bimwe na bimwe bya Chemical.Tris-HCl nayo yakoreshejwe nkurwego rwa titre.

Gutegura igisubizo:fata tris (hydroxymethyl) aminomethane hydrochloride nkumuti, fata amazi ya deionioni nkumuti, hanyuma ukoreshe igisubizo cyamazi ya HCl hamwe na 0.1mol / L kugirango uhindure agaciro ka pH kugirango utegure igisubizo cya trimethylolamine hamwe na 6.057g / L na pH = 8.8.Hydroxymethylaminomethane hydrochloride buffer igisubizo (buffer ya Tris-HCl).

Ibikoresho bya shimi:Kirisiti yera.

Ikoreshwa:PH ya buffers zose ni ubushyuhe hamwe nibitekerezo biterwa.Kuri buffer ya Tris, pH yiyongera hafi 0,03 kuri 1 ° C kwiyongera kwubushyuhe kandi igabanukaho 0,03-0.05 kuri 10 yikubye inshuro 10.

Gusaba:kuri buffer biologiya

Ikoreshwa:ikoreshwa nkumuhuza muri synthesis