Ibicuruzwa

Acide nshya ya zwitterionic butanesulfonic yagura alkaline yumuryango wimiryango ine ya buffer nziza: isuzuma ryo gukoresha muri biologiya.

Ibice bine bishya bya zwitterionic butanesulfonic acide ifitanye isano nimiryango ine ya buffers nziza yasuzumwe kugirango ikoreshwe mubinyabuzima.Izi buffers, zifite agaciro ka pKa kuva 7,6 kugeza 10.7, zagereranijwe nizindi mbuto zitandukanye zo mumuryango umwe hamwe na buffer zidafitanye isano kugirango bamenye ingaruka zabyo mubikorwa bya enzyme no gukura kwa mikorobe.Igikorwa cyimisemburo ine ifite agaciro keza pH murwego rwa alkaline yageragejwe: beta-galactosidase, esterase, fosifisiyose na fosifata ya alkaline.Muri rusange, buffers zose nziza, harimo na bffer nshya ya butanesulfonike, yatanze ibikorwa byiza;icyakora, habaye itandukaniro mubikorwa bya enzymes zimwe na buffers zimwe.Ububiko bwa Tris, glycine, na fosifate mubisanzwe byerekanaga itandukaniro mubikorwa ugereranije numuryango wa buffers nziza.beta-Galactosidase, byumwihariko, yerekanye ibikorwa byinshi hamwe na buffer nziza kuruta fosifate cyangwa Tris buffers.Mu buryo nk'ubwo, imikurire ya bagiteri irindwi yari ihamye, usibye bake, kumuryango mwiza wose wa buffers hamwe na Tris akenshi ibuza gukura.Umubare wa fosifata ya alkaline ihujwe na antibodies nigikoresho cyingenzi mubikorwa byinshi mubuzima bwa molekuline.Benshi mu bffers nziza batanze ibimenyetso byiza mugihe ugereranije na Tris kuri pH 9.5 kugirango bamenye poroteyine ukoresheje alkaline fosifata-conjugated antibodies.