Ibicuruzwa

Mu nyamaswa nyinshi zifite chromosomes X na Y igitsina, uburyo bwa chromosome bugari bukoreshwa muguhuza imvugo ya X ihuza igitsina gabo nigitsina gore.Isazi Drosophila melanogaster, uburyo bwo kwishyura dosiye nabwo muri rusange bugera kuri X.Imirongo irenga 70 ya transgenji yintama zo muri Ositaraliya Lucilia cuprina zakozwe murwego rwo gushyiraho ingamba zo guteza imbere ubwoko bwumugabo gusa muri gahunda yo kurwanya genetike yibi byonnyi byintama.Imirongo yose itwara fluorescent protein marike gene.Muri iyo mirongo 12 X yose ihuza, liswi yumugore yerekana fluorescence nziza kurusha liswi yumugabo, byerekana ko gene ya marike itari dosiye yishyuwe.Ibi byemejwe numubare RT-PCR kumirongo yatoranijwe.Kugirango tumenye niba endogenous X ihuza genes ari dosiye yishyuwe, twatandukanije genes 8 arizo orthologs ya genes ziri kuri chromosome ya kane muri D. melanogaster.Ibimenyetso biheruka kwerekana byerekana ko D. melanogaster ya chromosome ya kane, cyangwa Muller element F, ni chromosome ya basekuruza X muri Diptera yagarutse kuri autosome mu bwoko bwa Drosophila.Twerekana kuri PCR yuzuye ya ADN yumugabo nigitsina gore ko 6 kuri 8 ihuza itsinda F gen iba kuri X chromosome muri L. cuprina.Ibindi bice bibiri bya Muller F byagaragaye ko ari autosomal muri L. cuprina, mugihe genes ebyiri za Muller B zabonetse mukarere kamwe ka chromosome X na L. cuprina orthologs ya D. melanogaster Ephrin na gawky.Turabona ko L. cuprina X chromosome gen zigaragarira kimwe kubagabo nigitsina gore (ni ukuvuga dosiye yuzuye).Rero, bitandukanye na Drosophila, biragaragara ko sisitemu yindishyi ya dosiye ya Lucilia yihariye kuri gen endogenous ya X chromosome kandi ntishobora guhuzwa na transgene iherutse.