Ibicuruzwa

Poroteyine fosifora igenga imikorere itandukanye ya selile kandi igira uruhare runini mugukura hakiri kare ibimera.Kugira ngo twuzuze kandi twagure ku iperereza ryabanje ryakozwe na poroteyine ya fosifora mu ngemwe za Arabidopsis twakoresheje ubundi buryo buhuza gukuramo poroteyine mu bihe bitavuguruzanya hamwe na chromatografiya (IMAC) itunganijwe neza ya fosifore mu bice bya Rubisco-yabuze, hanyuma hakurikiraho kumenyekana ukoresheje gel-ibice bibiri bya gel electrophorei (2-DE) hamwe na chromatografiya y'amazi-tandem mass spectrometrie (LC-MS / MS).Muri-gel trypsin igogora no gusesengura ahantu hatoranijwe hagaragaye peptide 144 ya fosifori hamwe nibisigara, muri byo 18 gusa ni byo byabonetse muri fosifeti 4.0 na P3DB Arabidopsis thaliana ya fosifora yububiko.Kurenga kimwe cya kabiri cya 82 cyamenyekanye kuri fosifore yagize uruhare muri metabolisme ya karubone, fotosintezez / guhumeka cyangwa okiside itera imbaraga za smi.Gutunganyiriza fosifore nziza mbere ya 2-DE na LC-MS / MS bigaragara ko byongera imbaraga mu gutahura ibisigazwa bya fosifori ya threonine na tirozine ugereranije nuburyo bukoresha ubutunzi bwa peptide, byerekana ko ubwo buryo bwombi bwuzuzanya muburyo bwo gukwirakwiza urubuga rwa fosifori.Ugereranije ibisubizo ku ngemwe zikiri nto hamwe nizo zabonetse mbere kubibabi bya Arabidopsis bikuze byagaragaje poroteyine eshanu zifite fosifora zitandukanye muri utwo turemangingo, byerekana ubushobozi bwubu buhanga bwo gukora iperereza ku mikorere ya fosifori ya poroteyine mugihe cyo gukura kw'ibimera.