PMSF Cas: 329-98-6 98.0% ifu ya kirisiti yera ya phenylmethanesulfonyl fluoride (PMSF)
| Umubare wa Cataloge | XD90250 |
| izina RY'IGICURUZWA | Fenilmethanesulfonyl fluoride (PMSF) |
| URUBANZA | 329-98-6 |
| Inzira ya molekulari | C7H7FO2S |
| Uburemere bwa molekile | 174.1927 |
| Ibisobanuro birambuye | Ibidukikije |
| Amategeko agenga ibiciro | 29049900 |
Kugaragaza ibicuruzwa
| Suzuma | ≥98.0% HPLC |
| Kugaragara | ifu ya kirisiti yera |
PMSF ni serine idasubirwaho / proteine inhibitor ikoreshwa cyane mugutegura lysates.
Mu bushakashatsi bwa vitro: PMSF (2 mM) yabujije karubasi iterwa na inositol fosifate ikwirakwizwa na 15% -19% gusa imbere ya Li +.Kubuza ibicuruzwa bya fosifinositide na PMSF biterwa nintambwe imwe cyangwa nyinshi nyuma yo gusenyuka kwa fosifinositide [1].PMSF ibuza acylation ibisigisigi bya inositol bya GPI abahuza mumaraso ya T. brucei.PMSF ibuza glycolipide C ariko ntigire aside irike muri vitro.PMSF ibuza kongeramo acylation ya GPI na fosifata ya Ethanolamine muri trypanosomes ya procyclic, ariko ntabwo iri muri selile ya Hela [2].
Mu bushakashatsi bwa vivo: PMSF (0.1 mL / 10 g b.wt, ip) yabyaye antinociception, nkuko bigaragazwa no kwiyongera kwinshi kwa% MPE mugusuzuma ubukererwe bwumurizo, ariko ntibyashoboka gutanga moteri isobanutse neza.Imbeba zabonye inshinge za intraperitoneal za PMSF zerekanye ingaruka z'urumogi zirimo antinociception, hypothermia hamwe nubudahangarwa hamwe na ED50 ifite agaciro ka 86, 224 na 206 mg / kg.Kwiyitirira PMSF (30 mg / kg) byongera ingaruka za anandamide kubisubizo byumurizo (antinociception), ibikorwa bya lokomoteri no kugenda byikubye inshuro 5, inshuro 10 na 8 inshuro 8.
Ubushakashatsi bwinyamaswa: Imbeba za ICR zabagabo zipima 18 kugeza 25 g zakoreshejwe mubushakashatsi.PMSF yashongeshejwe mumavuta ya sesame hanyuma itangwa muburyo budasanzwe mubunini bwa 0.1 mL / 10 g b.wt.Buri gihe utange PMSF iminota 10 mbere yo gutera anandamide cyangwa gutera inshinge.Imbeba zamenyereye icyumba cyo gusuzuma ijoro ryose nta guhagarika ibiryo cyangwa amazi.Nyuma yubuyobozi bwa anandamide cyangwa imiyoborere yimodoka, buri nyamaswa yasuzumwe kuburyo bukurikira: iminota 5 yo gutinda umurizo (antinociceptive) ibisubizo niminota 5 kugeza kuri 15 kubikorwa byikora (moteri);cyangwa iminota 5 kubushyuhe (rectal) n'ubushyuhe Immobilisation (catalepsy) muminota 5 kugeza 10.
