Ibicuruzwa

Iriburiro: BSA ni imwe muri poroteyine zikoreshwa cyane muri laboratoire y’ibinyabuzima, kandi akamaro kayo karashobora kwirengagizwa mu bushakashatsi kuko ni ibisanzwe kandi ni mundane.Bovine serum albumin (BSA), izwi kandi nk'igice cya gatanu, ni globuline muri serumu ya bovine irimo ibisigisigi bya aside amine 583, ifite uburemere bwa molekile ya 66.430kDa hamwe na isoelectric point ya 4.7.BSA ifite uburyo bwinshi bwo gukoresha mubushakashatsi bwibinyabuzima, nkibikorwa byo guhagarika iburengerazuba.

Gusaba: Bovine Serum Albumin (BSA), izwi kandi nkigice cya gatanu, ni globuline muri serumu ya bovine, irimo ibisigisigi bya aside amine 607, kandi ifite uburyo bwinshi bwo gukoresha mubushakashatsi bwibinyabuzima.Bovine serum albumin isanzwe ikoreshwa nka stabilisateur mubisubizo byububiko hamwe nigisubizo cyibisubizo byimisemburo yabujijwe cyangwa imisemburo yahinduwe kugirango ihagarike ibikorwa bya enzyme kandi irinde kwangirika kwa enzyme hamwe na adsorption idasanzwe.

Imikorere: BSA isanzwe ikoreshwa nka stabilisateur mubisubizo byububiko nigisubizo cyibisubizo byimisemburo yabujijwe cyangwa imisemburo yahinduwe, kubera ko imisemburo imwe nimwe idahindagurika cyangwa ifite ibikorwa bike mubitekerezo bito.Nyuma yo kongeramo BSA, irashobora kugira uruhare rwa "kurinda" cyangwa "umutwara", kandi ibikorwa bya enzymes nyinshi birashobora kunozwa cyane nyuma yo kongera BSA.Enzymes zidasaba kongerwamo BSA mubusanzwe ntabwo ziterwa no kwiyongera kwa BSA.Kuri ADN nyinshi zifata ADN, BSCA irashobora gutuma igogorwa ryuzura, kandi rishobora gukata inshuro nyinshi.Kuri 37 ° C, iyo igogorwa ryigifu rirenze 1 h, BSA irashobora gutuma enzyme ihagarara neza, kuko muri buffer reaction idafite BSA, imisemburo myinshi yo kubuza irashobora kubaho kuri 37 ° C gusa 10 ~ 20min cyangwa mugihe gito..Ibinyuranye, BSA irashobora guhuza ioni nicyuma nindi miti muri buffer cyangwa substrate ADN ibuza ibikorwa byo kubuza endonuclease.

Imikoreshereze: Urwego rusanzwe rwa bovine serum albumin (BSA, StandardGrade), rushobora guhaza ibikenewe mubushakashatsi busanzwe, nka agent immunoblocking agent, selile selile (mikorobe yinyamanswa nudukoko, nibindi) intungamubiri zumuco nibigize umuco, protein / enzyme stabilisation reagents hamwe na protein igipimo cyo kugereranya.Indwara yo kwisuzumisha bovine serum albumin (BSA, DiagnosticGrade) irashobora guhaza ibyifuzo byinshi bisanzwe bikenerwa mubushakashatsi, nka agent immunoblocking agent, protein / enzyme stabilisateur, diluent, umutwara hamwe na protein.Byongeye kandi, irashobora kandi gukoreshwa mugukingira indwara, umuco w'utugari no kugerageza kuvanga bisaba sensibilité yo hejuru.