D-Luciferin Cas: 2591-17-5 99% Off-yera kugeza ifu yumuhondo nbsp BEETLE LUCIFERIN
| Umubare wa Cataloge | XD90248 |
| izina RY'IGICURUZWA | D-Lusiferi |
| URUBANZA | 2591-17-5 |
| Inzira ya molekulari | C11H8N2O3S2 |
| Uburemere bwa molekile | 280.323 |
| Ibisobanuro birambuye | -15 kugeza kuri 20 ° C. |
| Amategeko agenga ibiciro | 29342080 |
Kugaragaza ibicuruzwa
| Ibirimo Amazi | Icyiza.2.0% |
| Guhindagurika | Max 2.0 NTU |
| Guhinduranya neza | -36 kugeza -32 |
| Kugaragara | Ifu yera-ifu yumuhondo |
| HPLC | Min 99% |
| Coefficient yo kuzimya | min 17900 L / (mol cm) |
Iriburiro: D-luciferin ni substrate ya adenosine triphosphate (ATP) -bishingiye kuri bioluminescence reaction.Ihame rya bioluminescence nuko luciferine iba oxyde na luciferase imbere ya ATP na ogisijeni.Imiti ivura imiti niyi ikurikira: ATP + D-Luciferin + O2 → Oxyluciferin + AMP + PPi + O2 + Umucyo.
Uburyo bwibikorwa: Uburyo bwibikorwa bya D-luciferin ni uko mugikorwa cya ATP na luciferase, luciferine (substrate) ishobora okiside kugirango itange urumuri.Iyo luciferine irenze, umubare wa fotone yakozwe uhuza neza hamwe nubunini bwa luciferase.
Gushyira mu bikorwa: D-luciferin ni substrate ya adenosine triphosphate (ATP) -bishingiye kuri bioluminescence reaction.Imyitwarire ya bioluminescence ya luciferin / luciferase ikoreshwa kenshi mugutahura ATP, metabolite ishobora guhinduka muri ATP (nka AMP, ADP, cAMP), na enzymes zishobora kubyara ATP (nka creine kinase, nibindi), bityo. reaction ya bioluminescence irashobora gukoreshwa.Bikoreshwa mugushakisha ibintu byinshi byibinyabuzima.
Igikorwa cyibinyabuzima: D-Luciferin (Firefly luciferin) ni insimburangingo izwi cyane ya bioluminescence imbere ya ATP kugirango ikoreshwe mu mashusho ya bioluminescence ishingiye kuri luciferase hamwe na selile ishingiye cyane.
Mu bushakashatsi bwa vitro: D-luciferine ikorana na luciferase, ATP na ogisijeni kugira ngo itange urumuri, ibyo bikaba bigaragazwa na firime ifotora yoroheje kugira ngo igaragaze antibodiyide ziterwa na alkaline fosifata.
Mu bushakashatsi bwa vivo: gukoresha insimburangingo ya D-luciferin hamwe na luciferase yumuriro birinda ibibyimba byakira umubiri muburyo bwimbeba yimbeba ikingira kanseri yintanga, kuko gahunda ya bioluminescence yunvikana no gukura kwikibyimba kuruta amabwiriza yo kongera ibiro.
