Ibicuruzwa

1.Guhindura ibikorwa byimisemburo ifitanye isano na karubone ya hydroxyde metabolism ningirakamaro kubijumba bikonje bikonje (CIS).Igitabo cyitwa RING urutoki gene SbRFP1 cyarakoronijwe kandi imvugo yacyo yasanze idakonje cyane mubijumba byibirayi bya genotypes irwanya مۇستەقىل.Guhindura SbRFP1 mubirayi byemeje uruhare rwayo muguhagarika ibikorwa β-amylase na invertase, ibyo bikaba byadindije kwangirika kwa krahisi na sucrose no kwegeranya kugabanya isukari mubijumba bibitswe bikonje.Ibyavuye mu bushakashatsi byerekana neza ko SbRFP1 ishobora gukora nk'umugenzuzi mubi wa BAM1 na StvacINV1 kugirango yihutishe ikwirakwizwa ry’isukari mu gihe cy’ibirayi bya مۇستەقىل.

2.Intego yiki gikorwa yari iyo kwiga ingaruka zo guteka amavuta kuri pectin methylesterase (PME) hamwe nibikorwa bya endogenous α- na β-amylase mubice bitandukanye (cortex na pith) byibirayi bibisi kandi bivura ubushyuhe cv.Agria.Hatoranijwe ubushyuhe butatu butandukanye (55, 70 na 85 ° C).Kuri buri kigeragezo cyo guteka, imyirondoro yubushyuhe bwaranditswe kandi urugero rwo guteka rwagaragajwe mubijyanye no guteka. Guteka amamesa byagize uruhare runini muri PME kuri 55 ° C no kugabanya ibikorwa byayo mubushyuhe bwa nyuma bwo gutunganya (85 ° C) , hamwe numubare munini muri cortex (0.3745 ± 0.0007 µmol galacturonic aside (GA) g (-1) uburemere bushya (FW) min (-1)) ugereranije na pith (0.2617 ± 0.0012 µmol GA g (-1) FW min (-1)).Habaho kandi ubushyuhe-labile hamwe nubushyuhe butajegajega bwa isoforms ya PME mubice bifatwa nkibirayi.Kuvura ubushyuhe ukoresheje amavuta byatumye igabanuka rikabije ryibikorwa bya endogenous α- na β-amylase mubice byombi ugereranije na pota mbisi kugeza, nubwo nta gukuraho burundu.Imisemburo itesha agaciro ibinyamisogwe nayo wasangaga ikwirakwizwa muburyo butandukanye mubijumba mbisi. Guteka kumyanda byagize ingaruka muburyo butandukanye isuzumabushobozi ryibisigisigi bisigara mumyanya ifatwa yibirayi cv.Agria.Ubundi bushakashatsi burakenewe kugirango hemezwe ibisubizo byabonetse.