Ibicuruzwa

Isesengura rya proteome-yagutse ya fosifora yibintu biracyari ikibazo gikomeye cyo gusesengura kubera urusobe runini rwa poroteyine ya fosifora.Muri iki gikorwa, turasuzuma ubwuzuzanye bwa Lys-N, Lys-C, na trypsin bijyanye nubushobozi bwabo bwo gutanga umusanzu mu isesengura ryisi yose ya fosifore.Uburyo bunonosoye bwo guhanahana amakuru-pH akomeye yakoreshejwe mugutandukanya neza N-kurangiza acetylated, fosifora, na peptide idahinduwe.Hafi ya 5036 ya fosiforeptide idakabije irashobora kumenyekana hamwe nigipimo cyo kuvumbura ibinyoma cya <1% kuva mg 1 ya proteine ​​ukoresheje guhuza imisemburo itatu.Amakuru yacu yerekanaga ko guhuzagurika hagati yamakuru ya fosifopteptide yakozwe na protease zitandukanye byari marginal, mugihe guhuzagurika hagati yamakuru abiri asa nayandi makuru yabonetse yasanze byibuze inshuro 4 hejuru.Muri ubu buryo, gukoresha parallel ya Lys-N na trypsin byatumye ubwiyongere bwa 72% bwumubare wa fosiforeptide wamenyekanye ugereranije na d na trypsin wenyine, mugihe ubushakashatsi bwa trypsin bwigana gusa bwiyongereyeho 25%.Rero, mugihe twibanze gusa kuri data ya trypsin na Lys-N, twasanze 4671 fosifopeptide idakabije.Isesengura ryakozwe ku mbuga zagaragaye ryerekanye ko amakuru ya Lys-N na trypsin yatunganijwe mu buryo butandukanye cyane bwa fosifora, bikomeza kwerekana ko uburyo bwa Multrotease bufite agaciro kanini mu isesengura rya fosifore.