Ibicuruzwa

Polymer brush layer ishingiye kuri blok cololymers ya poly (oligo (Ethylene glycol) methacrylate) (POEGMA) na poly (glycidyl methacrylate) (PGMA) byakozwe kuri wafer ya silicon nabashinzwe gukora byakozwe na electron transfert atom transfert radical polymerisation (AGET ATRP).Ubwoko butandukanye bwa biomolecule burashobora guhurizwa hamwe murwego rwohanagura hifashishijwe reaction ya matsinda ya epoxide ya PGMA hamwe nitsinda rya amino muri biomolecule, mugihe POEGMA, irwanya poroteyine idafite akamaro, itanga ibidukikije.Ubuso bwaranzwe no guhuza amazi, ellipsometrie, na Fourier guhindura infragre spekitroscopi (FTIR) kugirango hemezwe impinduka.Gutandukanya icyiciro cya kopolymer mubice byarebwaga na AFM.Ingaruka yimiterere yubuso kuri protein conjugation yakozweho ubushakashatsi hakoreshejwe uburyo bwa radiolabeling.Byerekanwe ko ubuso bufite ibice bya POEGMA bwarwanyaga poroteyine, mugihe ubwinshi bwa poroteyine bwahujwe na diblock copolymer yahinduwe hejuru yiyongera hamwe no kongera umubyimba wa PGMA.Igikorwa cya lysozyme ihujwe hejuru nayo irashobora kugenzurwa muguhindura ubunini bwurwego rwa copolymer.Iyo biotine yahujwe nubukorikori bwa copolymer, ubuso bwakomeje kwihanganira poroteyine idafite akamaro ariko ikerekana avidin.Iyi mitungo, ni ukuvuga, igenzurwa neza nigikorwa cyibinyabuzima byahujwe na biomolecules hamwe nuburyo bwihariye bwimikoranire hamwe na biomolecules bigamije gukoreshwa kugirango habeho kunoza igipimo cyerekana-urusaku mubikorwa bya sensor.Mubisanzwe, isura nkiyi irashobora kuba ingirakamaro nkibintu byo kumenyekanisha ibinyabuzima byihariye biomateriali ikora.