Streptomycine sulfate CAS: 3810-74-0 Ifu yera
| Umubare wa Cataloge | XD90361 |
| izina RY'IGICURUZWA | Streptomycine sulfate |
| URUBANZA | 3810-74-0 |
| Inzira ya molekulari | (C21H39N7O12) 2 · 3H2SO4 |
| Uburemere bwa molekile | 1457.39 |
| Ibisobanuro birambuye | 2 kugeza 8 ° C. |
| Amategeko agenga ibiciro | 29412080 |
Kugaragaza ibicuruzwa
| Icyiciro | USP36 / EP8.0 |
| pH | 4.5-7.0 |
| Gutakaza Kuma | ≤7.0% |
| Suzuma | 20720IU / MG |
| Sulfate | 18.0 ~ 21.5% |
| Kugaragara | Ifu yera cyangwa hafi yera, gushonga cyane mumazi, hafi yo kudashonga muri Ethanol. |
| Methanol | ≥0.3% |
| Streptomycin B. | ≤3.0% |
| Ikizamini cya Colorimetric | ≥90.0% |
| Acide ya sulfure | ≤1.0% |
| Ibirimo bya sodium sulphite | ≤0.4% |
Nubwo etiologiya yo kwangirika kwa disikete itumvikana neza, inzira imwe yo gukumira iki gikorwa irashobora kuba iyo kubuza apoptose.Mu bushakashatsi burimo gukorwa, ubushakashatsi bwakozwe na anti-apoptotique ya chitosani ya carboxymethylated chitosan (CMCS) muri selile nucleus pulposus (NP) hagamijwe kuzamura ubuzima bwa selile.Ingirabuzimafatizo za NP zashyizwe mu bwigunge kandi zifite umuco muri vitro, kandi hydrogen peroxide (H2O2) yakoreshejwe mu kubaka icyitegererezo cya NP selile apoptose.Ubushobozi bwakagari bwasuzumwe hamwe nibikoresho byo kubara selile - 8 assay.Umubare w'ingirabuzimafatizo za apoptotique wakozweho ubushakashatsi na annexine V - fluorescein isothiocyanate (FITC) na propidium iodide (PI) isesengura ryikubye kabiri, kandi morphologie yagaragaye na Hoechst 33342.Mitochondrial membrane ubushobozi bwa selile NP yasuzumwe na rhodamine 123 fluorescence yanduye.Guhindura transcription (RT) ‐quantitative polymerase reaction reaction (qPCR) byakozwe kugirango bapime urugero rwa mRNA ya synthase ya nitric oxyde ya nitric (iNOS), caspase - 3, B - lymphoma selile (Bcl) ‑2, ubwoko bwa II kolagen na aggrecan.Isesengura ryiburengerazuba ryakozwe kugirango hamenyekane urwego rwa poroteyine za iNOS na Bcl - 2.Umugereka wa V - FITC / PI na Hoechst 33342 ibisubizo byerekana ko CMCS yashoboye gukumira selile NP kwandura apoptose muburyo bukabije.Rhodamine 123 yerekana neza ko CMCS yagabanije kwangirika kwa mitochondrial membrane ishobora kuba muri H2O2 - ivura selile NP.Kugabanya caspase - 3 no kongera ibikorwa bya Bcl - 2 byagaragaye muri CMCS - bivura selile NP na RT - qPCR hamwe nisesengura ryiburengerazuba.CMCS yateje imbere kandi ikwirakwizwa ryubwoko bwa II kolagen na aggrecan muri H2O2 - ivura selile NP.CMCS yagaragaye ko ifite akamaro mukurinda urupfu rwa selile apoptotique muri vitro, byerekana ibyiza bishobora guterwa nubu buryo bwo kuvura muguhindura disiki.
