Ibicuruzwa

ExPortal protein isohora organelle muri Streptococcus pyogène ni anionic fosifolipide irimo membrane microdomain ikungahaye kuri Sec translocons hamwe na proteine ​​biogenezi ya postsecretion.Polymyxin B ihuza kandi igahungabanya ubunyangamugayo bwa ExPortal, bikavamo gusohora inenge uburozi bwinshi.Kugirango usobanukirwe nibintu bigira ingaruka kumuryango wa ExPortal, hakozwe ecran ya geneti kugirango ihitemo ihinduka ryimiterere ya polymyxine B irwanya ihinduka ryerekana ubunyangamugayo bwa ExPortal.Ubwoko bwa genome buringaniye bwa mutant 25 irwanya ihinduka ryagaragaye kuva kuri mutation imwe kugeza kuri enye kuri genome ya mutant ihuriweho cyane cyane mubice bigize amatsinda 10 ya gene.Kubaka mutant hamwe no gusiba kugiti cye cyangwa kwinjiza byerekanaga ko ingirabuzimafatizo 7 zitanga imbaraga kandi zikongerera ubunyangamugayo ExPortal binyuze mu gutakaza imikorere, mugihe 3 bishoboka ko byatewe no kunguka imikorere hamwe na / cyangwa guhuza imbaraga.Ingirabuzimafatizo zikomeye zirimo kugenzura imiterere ya lipide biosynthesis, genes nyinshi zigira uruhare mu gushaka intungamubiri, hamwe na genes zitandukanye zigira uruhare mu gukemura ibibazo.Babiri mubagize icyiciro cya nyuma nabo bakora nkabashinzwe kugenzura udushya twa protease yihuta ya SpeB.Isesengura ryimiterere ihindagurika cyane, gusiba nucleotide imwe mumurongo wibisigisigi 9 bikurikiranye bya adenine muri pstS, bikubiyemo igice cyumuntu utwara Pi cyane, byerekana ko uru ruhererekane rukora nka molekile kugirango byorohereze imihindagurikire.Hamwe na hamwe, aya makuru yerekana ko habaho igisubizo gihangayikishije giteza imbere ubunyangamugayo bwa ExPortal no kurwanya peptide ya anticicrobial cationic.