Ibicuruzwa

Ubu bushakashatsi busobanura uburyo bworoshye kandi bunoze bukoresha imikorere ya chromatografiya ikora cyane (HPLC) ihujwe no gutahura fluorescence kugirango hamenyekane ibipimo bya kinetic yo gufata glutamate mumyanya mitsi.Utugingo ngengabuzima twakuwe mu nkoko zimaze iminsi 7 zashyizwemo ubushakashatsi buzwi bwa glutamate (50-2000 μM) mu minota 10, hanyuma hapimwa urwego rwa o-phtaldehyde (OPA) -gutanga imbaraga za neurotransmitter mu buryo bwa incubation.Mugusuzuma itandukaniro riri hagati yubushakashatsi bwambere nubwa nyuma bwa glutamate hagati, uburyo bwo gufata ibintu bwuzuye bwaranzwe (K (m) = 8.2 na V (max) = 9.8 nmol / mg protein / min).Iki gipimo ahanini cyari gishingiye kuri sodium- nubushuhe bushingiye ku bushyuhe, bushigikira cyane ko uburyo bwo kugabanya ibitekerezo byatewe rwose nabatwara abantu benshi.Twongeyeho kuri ibi, ibisubizo byacu byerekanaga kandi ko zinc chloride (inhibitori ya glutamate / abatwara ibintu) yatumye igabanuka rishingiye ku kwibanda ku kugabanuka kwa glutama te, byerekana umwihariko w'uburyo bwacu.Muri rusange, imirimo iriho iranga ubundi buryo bwo gusuzuma glutamate mu myanya myakura ikoresheje HPLC.Ubu buryo bushobora kuba igikoresho cyingenzi cyubushakashatsi bujyanye no kuranga iminota ihindagurika muri transport ya glutamate ijyanye no gukomeretsa kwa sisitemu yo hagati.