Ibicuruzwa

Poroteyine ya poroteyine ya serine / threonine yasobanuwe muri bagiteri nyinshi zitera indwara nka enzymes zingenzi zigira uruhare mu nzira yo kwanduza ibimenyetso bya fosifora kandi akenshi bifitanye isano na virusi yibinyabuzima.Ubushakashatsi bwakozwe na genoside ya Mycoplasma synoviae bwerekanye ko hariho gene (prpC) ikubiyemo poroteyine fosifata ya poroteyine fosifata 2C (PP2C) mu buryo butemewe.Hano, twatanze raporo yuzuye ya biohimiki iranga M. synoviae phosphatase (PrpC) nuruhare rwihariye rwa ioni ibyuma muburyo bwimikorere-imikorere yiyi misemburo.Isesengura rya aside amine ya PrpC ryerekanye ko ibisigazwa byose bigira uruhare mu kigo cy’icyuma cya kirimbuzi hamwe n’ibisigazwa bya gatatu bya ion bihuza ibisigazwa, bibitswe muri fosifata ya PP2C, biboneka muri PrpC.PrpC ni poroteyine ya monomeric ishoboye dephosifora ya fosifore-substrate hamwe na ion ya Mn (2+).Isesengura ryubushyuhe bwumuriro ryerekanye enzyme ihamye kubushyuhe bworoheje hamwe na inf luence ya Mn (2+) muri uyu mutungo.Isesengura rusange ryerekana ko ion eshatu zicyuma zihuza na PrpC, ebyiri muri zo zikaba zigaragara cyane-zihoraho.Isesengura ry’imihindagurikire y’ibisigazwa bya gatatu byashyizwe mu bikorwa, Asp122 na Arg164, byagaragaje ko izo mpinduka zagaragaje intege nke za ion ya manganese, kandi ko ihinduka ryombi ryagize ingaruka ku bikorwa bya fosifata ya PrpC.Ukurikije ibisubizo, PrpC ni umunyamuryango wa poroteyine fosifata iterwa nicyuma kandi ifite umutekano muke muburyo bwa holo hamwe na Asp122, birashoboka ko yaba ifite umwanya wa gatatu uhuza ibyuma, byingenzi mubikorwa bya catalitiki.