Proteinase K ya alubumu ya tritirachium Cas: 39450-01-6 99% Ifu yera
| Umubare wa Cataloge | XD90434 |
| izina RY'IGICURUZWA | Proteinase K ya alubumu ya tritirachium |
| URUBANZA | 39450-01-6 |
| Inzira ya molekulari | C29H27N2O12P |
| Uburemere bwa molekile | 626.50468 |
| Ibisobanuro birambuye | -15 kugeza kuri 20 ° C. |
| Amategeko agenga ibiciro | 35079090 |
Kugaragaza ibicuruzwa
| Uburemere bwihariye | 40un / mg protein min |
| Igikorwa cya Enzyme | 30un / mg min uburemere bwumye |
| Kugaragara | Ifu yera |
| Suzuma | ≥99% |
Ubushakashatsi bwakozwe na chromatin immunoprecipitation (ChIP) bwazanye ubushishozi bwerekeranye na genomicale ya poroteyine zifitanye isano na chromatine hamwe na histone ihinduka.Umubare munini wamakuru yatanzwe niri sesengura, ariko, arasaba inzira zituma byemezwa byihuse nisesengura ryibinyabuzima.Byongeye kandi, haracyari intego za poroteyine noguhindura bigoye kumenya ukoresheje uburyo busanzwe bwa ChIP.Kugira ngo dukemure ibyo bibazo, twashyizeho uburyo bwihuse bwa chromatin immunoprecipitation twita ZipChip.ZipChip igabanya cyane igihe kandi ikongera ibyiyumvo byemerera kwerekanwa byihuse bya loci nyinshi.Hano turasobanura uburyo ZipChIP ituma hamenyekana ibyahinduwe na histone (H3K4 mono- na trimethylation) hamwe na demethylase yimisemburo ibiri, Jhd2 na Rph1, byari bigoye kubimenya hakoreshejwe uburyo busanzwe.Byongeye kandi, twerekana impinduramatwara ya ZipChIP dusesenguye itunganywa rya histone deacet ylase Sir2 kuri heterochromatine mumusemburo no gutunganya chromatin remodeler, PICKLE, kuri euchromatin muri Arabidopsis thaliana.
