Ibicuruzwa

3-Morpholinepropanesulfonic aside ni ibinyabuzima kandi ikoreshwa kenshi mugutegura amashanyarazi ya RNA.

Imiterere yo kubika:3-Morpholinepropanesulfonic aside ni aside sulfonike, kandi igomba kubikwa ku bushyuhe bwicyumba, kure yumucyo nubushuhe.Igikorwa cyibinyabuzima: MOPS isanzwe ikoreshwa nka buffer muri biologiya.MOPS buffer ikomeza pH yibitangazamakuru byumuco winyamabere.

Gusaba:Buffer ya biologiya, ikoreshwa mubikoresho byo gusuzuma biohimiki, ibikoresho byo gukuramo ADN / RNA nibikoresho byo gusuzuma PCR

Ikoreshwa:Ibigize muri Buffer Nziza kubushakashatsi bwibinyabuzima

MOPS na coxsackievirus B3 ituze

Ubushakashatsi bwakozwe na coxsackievirus B3 bugoye 28 (CVB3 / 28) butajegajega ukoresheje MOPS mugutezimbere bufferi muburyo bwubushakashatsi bwerekanye ko MOPS (3-morpholinopropane-1-sulfonique acide) yongereye CVB3 ituze kandi ingaruka zishingiye ku kwibandaho.Kurenza pH 7.0-7.5, kwandura virusi byatewe no kwibanda kuri pH na MOPS.Docking ya mudasobwa yakozwe na mudasobwa yerekanaga ko MOPS ishobora gufata umufuka wa hydrophobi muri capsid protein VP1 aho itsinda rya acide sulfonique rishobora gukora ionic na hydrogen hamwe na Arg95 na Asn211 hafi yo gufungura umufuka.Ingaruka za MOPS hamwe na hydrogène ion yibanze ku gipimo cyo kwangirika kwa virusi byagereranijwe no gushyiramo ibipimo bijyanye na kinetic ya vuba.Ibisubizo byerekana ko MOPS ishobora guhuza na CVB3 no guhagarika virusi, bishoboka muguhindura capsid ihuza imbaraga.