Ibicuruzwa

Bicine ni buffer ya zwitterionic amino acide, ikora murwego rwa pH 7.6-9.0 (pKa ya 8.26 kuri 25 ° C).Basabwe buffer kubushyuhe buke bwibikorwa bya biohimiki.Bicine ikoreshwa mugutegura igisubizo gihamye cyibisubizo bya serumu guanase.Ikoreshwa rya bicine muburyo bworoshye ion yo guhana chromatografiya yo gukemura poroteyine yashyizwe ahagaragara.Bicine yakoreshejwe muri peptide na protein korohereza.Ubushakashatsi bwa kinetic yinzibacyuho-leta analogue igizwe na creatine kinase yakoresheje bicine muri reaction buffer.Sisitemu ya buffasike ya SDS-PAGE ya poroteyine na peptide zirimo bicine byasobanuwe.

Urukuta rumwe rwa alveolar rwakorewe ubushakashatsi burebure muri saline na Bicine (0.2 M) bigenda byangirika buhoro buhoro mu guhagarika ingirabuzimafatizo (TTD).Twasuzumye ingaruka zibisubizo bitandukanye kuriyi TTD kandi dushakisha impinduka zijyanye na ultrastructure.Ibihaha parenchyma yajugunywe ku rukuta rumwe rwa alveolar (30 X 30 X 150 microne) muri saline ya fosifate (0.15 M).Yimuriwe mu bwogero burebure, tissue yinjijwe muri Bicine, saline, ishimangira igisubizo cya Hank, 0.25% ubururu bwa Alcian muri saline, cyangwa igisubizo cya sodium dodecyl sulfate, mubihe bitandukanye.Kuzenguruka binyuze mu kwaguka gutangwa n'imbaraga zo hejuru zapimwe mugihe, izo nyama zimwe zashyizwe muri acide glutaraldehyde / tannic acide hanyuma itunganyirizwa kuri microscopi electron.Urukuta rumwe rwa alveolar rwinjijwe muri saline cyangwa Bicine rwerekanye TTD igenda itera imbere.Vacuole cyangwa umwanya byagaragaye muri interstitium hamwe na selile selile selile yateye imbere hamwe na TTD.Urebye muri 0.3 h, impinduka zateye imbere neza kuri 0,6 h.Muri sodium dodecyl sulfate (mM 70), ariko, nta TTD yari ihari kandi muburyo nta interstitium yari ihari, hasigaye gusa ibyumba byo hasi na proteine ​​fibrous.Mu gisubizo gikomeye cya Hank cyangwa 0,25% Ubururu bwa Alcian matrice interstitial, morphologie selile hamwe nuburemere bwimitsi yabitswe neza kuri 1 h.Ubu bushakashatsi bwerekana ko gutobora matrice interstitial biboneka muri saline cyangwa Bicine, kandi matrike idakenewe ningirakamaro mu kurinda impagarara.