Ibicuruzwa

Igituntu cya Mycobacterium kirashobora kumara imyaka mirongo mubakira abantu.Inzira zikomeye zo gusubiza zirimo polyphosphate [poly (P)], ikomatanyirizwa hamwe na hydrolyz na polyphosphate kinase (PPK) na exopolyphosphatase (PPX), bigira uruhare runini mugukurikiza bagiteri.Twerekanye hano ko M. igituntu poly (P) kwirundanya bifitanye isano nigihe gito no gukura kwingingo.Tumenye kandi M. igituntu Rv1026 nkigitabo gishya cyitwa exopolyphosphatase hamwe nibikorwa bya hydrolytique birwanya poly ndende (P).Twifashishije sisitemu ya tetracycline-idasobanutse kugirango ikureho imvugo ya Rv1026 (ppx2), twasanze kwirundanya kwa M. igituntu (P) biganisha ku gukura gahoro no kugabanya kwandura isoniazide, kongera imbaraga zo kurwanya ubushyuhe na aside pH, no kongera ubuzima bwo mu nda. mugihe cyo kwandura macrophage.Mu kwanduza microscopi electronique, ppx2 knockdown yerekanaga ubukana bwurukuta rwakagari, ibyo bikaba byari bifitanye isano no kongera urukuta rwa selile rwinjira mumiti ya hydrophilique aho kwinjiza pompe yibiyobyabwenge cyangwa guhindura biofilm ugereranije no kugenzura ubusa.Isesengura rya Transcriptomic na metabolomic ryagaragaje metabolike igabanuka rya ppx2 gukomanga kurangwa no kugabanuka kwandikirwa no guhindura hamwe no kugabanuka kwa glycerol-3-fosifate.Muri make, poly (P) igira uruhare runini mukugabanya imikurire ya M. igituntu no kugabanuka kwa metabolike kandi ikagira uruhare mu kwihanganira antibiyotike binyuze mu rukuta rw’imitsi rwahinduwe. Igisubizo gikaze, kirimo molekile zigenga imiterere ya polifosifike [poly (P)] na (p ) ppGpp, byizerwa guhuza Mycobacterium igituntu gikomeza.Muri ubu bushakashatsi, twabonye enzyme nshya (Rv1026, PPX2) ishinzwe hydrolyzing poly-ndende ndende (P).Ubwoko bwa M. tuberculose bwakozwe na genetike yabuze gene ya ppx2 bwerekanye urugero rwinshi rwa poly (P), ibyo bikaba byarajyanye no gufatwa kwa bagiteri hakiri kare kandi bikagabanya kwandura indwara ya isoniazid yo ku murongo wa mbere, ndetse no kongera ubuzima bwa bagiteri mu gihe cyo guhangayika imiterere no muri macrophage.Ugereranije nuburyo bwo kugenzura, mutant yerekanye ubwiyongere bwurukuta rwakagari kandi bigabanya ibiyobyabwenge.Imvugo ya gene yisi yose hamwe nisesengura rya metabolite byagaragaje imvugo igabanya imashini zandikirwa hamwe noguhindura no guhindura imikoreshereze ya karubone.Muri make, kugenzura ibipimo bya poly (P) nibyingenzi kugirango habeho gukomera muri M. igituntu.