Ibicuruzwa

Poroteyine ya ORF45 ya Kaposi ya sarcoma ifitanye isano na herpesvirus (KSHV) ni gammaherpesvirus yihariye proteine ​​ya proteine.Ubushakashatsi bwatubanjirije bwerekanye uruhare rukomeye haba hakiri kare na nyuma yo kwandura KSHV.Muri ubu bushakashatsi, twasesenguye imikoranire ya ORF45 dukoresheje akanama ka antibodiyite za monoclonal.Usibye kumenyekana mbere ya kinase idasanzwe (ERK) na p90 ribosomal S6 kinase (RSK), twasanze izindi poroteyine nyinshi zifite kopi, harimo izingenzi za ∼38 kDa na 30130 kDa.Mass spectrometrie yerekanye ko poroteyine 38-kDa ari virusi ORF33 naho poroteyine 130-kDa ni selile USP7 (protease yihariye ya ubiquitin 7).Twashushanyije indangarubuga ya ORF33 ihuza carboxyl-terminal yabitswe cyane na aminide acide 19 (aa) ya ORF45 hamwe na USP7 ihuza domeni kubitekerezo byavuzwe mubwumvikane mukarere ka ORF45.Twifashishije irangi rya immunofluorescence, twabonye colocalisation ya ORF45 hamwe na ORF33 cyangwa USP7 haba munsi ya ion ya kanseri yanduye ndetse no muri selile zanduye KSHV.Byongeye kandi, twabonye kwimura ORF45 biterwa no kwimura igice cya ORF33 / USP7 kuva muri nucleus kugera kuri cytoplazme.Twabonye ko ORF45 yatumye kwiyongera kwa poroteyine ya ORF33 byavanyweho niba domeni ya ORF33- cyangwa USP7 ihuza ORF45 yasibwe.Byongeye kandi, gusiba carboxyl terminus yabitswe ya ORF45 muri genoside ya KSHV byagabanije cyane urwego rwa poroteyine ya ORF33 mu ngirabuzimafatizo zanduye KSHV kandi bivanaho umusaruro wa virusi.Hamwe na hamwe, ibisubizo byacu ntibigaragaza gusa ibice bishya bigize imikoranire ya ORF45, ahubwo binerekana ko imikoranire hagati yizo poroteyine ari ingenzi cyane mu kwigana lytike ya KSHV.Kaposi ya sarcoma ifitanye isano na herpesvirus (KSHV) ni yo nyirabayazana ya kanseri nyinshi z’abantu.KSHV ORF45 ni poroteyine ikora cyane isabwa kugirango KSHV yigane, ariko uburyo nyabwo ORF45 ikora imirimo yayo ikomeye ntibisobanutse.Ubushakashatsi bwatubanjirije bwerekanye ko ORF45 pr otein yose muri selile ibaho murwego rwo hejuru-rufite uburemere.Twashatse rero kuranga imikoranire ya ORF45 kugirango tumenye uruhare rwayo mugihe cyo kwigana.Twifashishije akanama ka antibodiyite za monoclonal, twasesenguye imikoranire ya ORF45 muri selile zanduye KSHV.Twabonye abafatanyabikorwa babiri bashya ba ORF45: poroteyine ya virusi ORF33 na selile ubiquitin yihariye protease 7 (USP7).Twongeye kwerekana ko imikoranire hagati ya ORF45 na ORF33 ari ingenzi cyane kugirango habeho umusaruro mwiza wa virusi ya virusi ya KSHV, byerekana ko kwivanga kugamije gukorana bishobora kwerekana ingamba nshya zo kubuza kwigana KSHV.