Ibicuruzwa

Kugirango tumenye neza metabolite nyinshi ya 5-fluorouracil (5-FU) na nucleotide ya endogenous monophosphate, twateje imbere uburyo bwumwimerere bushingiye kumazi ya chromatografiya - tandem mass spectrometrie (LC-MS / MS).Ubu bushakashatsi bwatanze icyemezo cyo: ,. 5-FU kuva selile zumuco zabantu nabatwara ABC.Mubihe byubushakashatsi bwacu, selile zashyizwemo 5-FU cyangwa 5-FUrd.Hanyuma, poroteyine ngengabuzima zajugunywe na methanol.Ubu buryo bwatanze umusaruro mwinshi.Twongeyeho, gupima 5-FU na 5-FdUMP isohoka mu tugari, twakoze umubare wa molekile mu muco.Ibitangazamakuru byatewe inshinge (5-FU) cyangwa munsi byagiye bivanwa mucyiciro cya karitsiye yo gukuramo Oasis Wax (5-FdUMP).Gutandukanya analyite byakorewe kuri dC18 Atlantis 3.5 mm, (100 mm × 2,1 mm id) inkingi hamwe nuburyo bwa gitewokarasi ukoresheje ammonium formate buffer / methanol / amazi (5/5/90, v / v) nkicyiciro kigendanwa.Igihe cyo kwiruka nticyarenze 6.2 min.Isesengura ryerekanwe muri interineti ya electrospray muburyo bubi bwa ion.Twemeje uburyo hejuru ya 2.5-150 ng mL −1 dukurikije ibice.Impinduka hagati-na-assay ihinduka yari munsi ya 10% muminsi irindwi.Ibicuruzwa byose byari bihamye mu ngirabuzimafatizo cyangwa mu muco wo hagati igihe ingero zabikwaga kuri −20 ° C byibura ibyumweru bibiri, na nyuma y’ibihe bitatu bikonje.Nta ngaruka ya matrix yagaragaye mubitangazamakuru byombi.