DCTP, 2′-Deoxycytidine-5′-triphosifori acide disodium umunyu Cas: 102783-51-7 Ifu yera;
| Umubare wa Cataloge | XD90584 |
| izina RY'IGICURUZWA | DCTP, 2'-Deoxycytidine-5'-triphosifori acide disodium umunyu |
| URUBANZA | 951-78-0 |
| Inzira ya molekulari | C9H12N2O5 |
| Uburemere bwa molekile | 228.20 |
| Ibisobanuro birambuye | -20 ° C. |
| Amategeko agenga ibiciro | 29349990 |
Kugaragaza ibicuruzwa
| Kugaragara | Ifu yera |
| Suzuma | 99% |
Ingirabuzimafatizo za Eukaryotique zirimo umunzani wuzuye wa minisiteri enye ya deoxyribonucleoside triphosphates (dNTPs), ihagije gusa muminota mike yo kwigana ADN.Byombi kubura hamwe nibisigisigi bya DNTP imwe bishobora gutuma habaho ihinduka ryimiterere ya mutation, gusana ADN nabi cyangwa kugabanuka kwa ADN ya mitochondial.DNTPs isanzwe igereranywa nubushakashatsi bwimikorere aho kwinjiza dATP ya radioaktike (cyangwa radiyo ikora dTTP mubisubizo bya dATP) muri oligonucleotide ya syntetique yihariye na polymerase ya ADN ihwanye nubushakashatsi bwa DNTP butazwi.Turabona ko polymerase ya Klenow ikoreshwa cyane irashobora gusimbuza ribonucleotide ihuye na dNTP itazwi iganisha mubihe bimwe na bimwe gukabya gukabije kwa DNTP.Ubu turasobanura imiterere ya buri dNTP irinda kwinjiza ribonucleotide.Kuri dTTP na dATP ivuga ko bihagije kugabanya ubukana bwa enzyme ya Klenow hamwe na dATP (cyangwa dTTP);kuri dCTP na dGTP twagombaga gusimbuza enzyme ya Klenow hamwe na polymerase ya Taq ADN cyangwa Thermo Sequenase.Turasaba ko muri raporo zimwe zabanjirije iyinjizwa rya ribonucleotide ishobora kuba yarateje agaciro gakomeye kuri dGTP na dCTP.
