Ibicuruzwa

Vuba aha twatangaje ko pyrene nucleotide yinjizwa muburyo butandukanye n’urubuga rwabasuzuguritse, 3'-T ya thymine dimer, hamwe n’ibishingwe byinshi bitarangiritse byumusemburo ADN polymerase eta (pol eta).Kuberako pyrene ari molekile idafite inkingi idafite ubushobozi bwa H-ihuza, imikorere idasanzwe idasanzwe yo kwinjiza dPMP yiswe ubushobozi bwayo bwo hejuru bwo gutondekanya, kandi ishimangira akamaro ko gutondekanya shingiro muguhitamo nucleotide na pol eta.Kugira ngo dukore iperereza ku ruhare rwa H-guhuza hamwe na geometrie fatizo mu gutoranya nucleotide na pol eta, twagennye uburyo bwo kwinjiza nucleotide yibanze-2,6-diaminopurine, 2-aminopurine, 6-chloropurine, na inosine yabikora kora umubare utandukanye wa H-bonds hamwe nicyitegererezo shingiro bitewe na geometrie ishingiro.Ihuriro rya Watson-Crick risa nkaho rifite uruhare runini muguhitamo ibigereranyo bya nucleotide kugirango byinjizwemo na C na T nkuko bigaragazwa no kugabanuka kwingirakamaro zijyanye no kwinjiza hamwe no kugabanuka kwumubare wa Watson-Crick H no kwiyongera kwa umubare w'abaterankunga-abaterankunga hamwe n'abemera-imikoranire.Guhitamo kwinjiza nucleotide birarenze bitandukanye na 5'-T kuruta 3'-T ya thymine dimer, ukurikije imirimo yabanjirije iyi yerekana ko 5'-T ifatwa cyane kuruta 3'-T.Byongeye kandi, kwinjiza A bitandukanye na Ts zombi za dimer bigaragara ko zahujwe no guhuza base ya Watson-Crick ntabwo ari uguhuza kwa Hoogsteen gushingiye kubikorwa byo kwinjiza hafi ya A na 7-deaza-A, ibyanyuma bikabura H- ubushobozi bwo guhuza kuri N7.Ingaruka zifatika zo kwinjiza nucleotide zishobora gukora ibice bibiri bya Watson-Crick bigereranywa nibice bya Klenow, mugihe igice cya Klenow kivangura cyane kudahuza, ukurikije uburyo bwacyo bwo guhitamo.Ibisubizo bishimangira akamaro ka H-guhuza hamwe na Watson-Crick base base geometrie muguhitamo nucleotide byombi pol eta nigice cya Klen ow, hamwe nuruhare ruto rwo guhitamo imiterere mugushyiramo na pol eta bitewe nuko ifunguye kandi nkeya urubuga rukora.