Ibicuruzwa

Inzira ya molekuliyumu itera indwara zumwijima cirrhotic nka virusi ya hepatite C (HCV), autoimmune hepatitis (AIH) na cirrhose yibanze ya biliary (PBC) irangwa nabi.Ingirabuzima fatizo zigaragazwa mu buryo butandukanye akenshi ni ingenzi mu gutera indwara.Gukuraho subtractive hybridisation (SSH) nuburyo bwa genome-bugari butungisha inyandiko zitandukanye za mRNA.Twari dufite intego yo gukora ubushakashatsi bushya bwerekana imiterere itandukanye ya gene muri cirrhose dukoresheje SSH ihujwe numubare nyawo wigihe-uhindura transcriptase polymerase urunigi (RT-PCR).Isuzuma ry'umwijima muri HCV cirrhose, AIH cirrhose, PBC, hamwe na tissue y'umwijima idafite uburwayi byasuzumwe na SSH.Ibisubizo byuzuzanya bya ADN (cDNA) byahinduwe kugirango bisobanurwe bitandukanye na dot-blot ivanga hanyuma bikurikiranwe.Imvugo yatoranijwe yagereranijwe nigihe-RT-PCR.Nyuma ya SSH, clone 694 zarasubitswe kugirango imvugo itandukanye ya gene itandukanye, muri zo 145 zikurikiranye kandi zisanga zikomoka kuri genes 89 zitandukanye.Clone zirindwi zari homologique gusa hamwe nurutonde rwikurikiranya (EST) rukurikirana genes zidafite umurimo uzwi.Kugaragaza neza imiterere ya genes enye byemejwe nigihe nyacyo RT-PCR: transmembrane 4 umunyamuryango wa superfamily 3 (tetraspanin CO-029) muburyo bwose bwa cirrhose, inzoka zikorana na poroteyine (HIP) muri AIH cirrhose na chitinase 3-nka-1 (HC gp-39 cyangwa ykl-40) na aside arginine-glutamic isubiramo (RERE) muri cirrhose ya HCV.RERE polymorphism hamwe na variice variants byagaragaye mubice byose byasuzumwe.Tetraspanin CO-029 up-amabwiriza yashizwe mbere na mbere na selile ductular selile.Mu gusoza, ubushakashatsi bushya bwerekana imiterere ya gene itandukanye muri cirrhose yabantu bwakozwe hakoreshejwe SSH nkigikoresho cyambere cyo kuvumbura.By'umwihariko, ubushakashatsi bwakozwe kuri gen RERE n'ibicuruzwa byayo muri HCV bifitanye isano n'indwara y'umwijima biremewe.