Ibicuruzwa

Brucellae ni etiologiya ya brucellose, zoonose ikwirakwizwa kwisi yose.Izi bagiteri ni parasite zo mu bwoko bwa parasite zifite ubushobozi bwo guhindura metabolisme kubidukikije bidasanzwe ndetse n’imitsi idasanzwe ihura nigihe cyanduye.Nyamara, iyi ngingo ya biologiya ya Brucella irasobanutse neza, kandi intungamubiri ziboneka muri niche zo mu nda ntizwi.Hano, twasesenguye inzira nkuru ya C metabolism yakoreshejwe na Brucella abortus dusiba fructose-1,6-bisphosphatase (fbp na glpX), phosphoenolpyruvate carboxykinase (pckA), pyruvate phosphate dikinase (ppdK), na enzyme ya malic (mae). genes.Muri gluconeogenic ariko ntabwo iri mubitangazamakuru bikize, imikurire ya ΔppdK na Δmae ihinduka ryangiritse cyane kandi imikurire ya mutant Δfbp-ΔglpX yagabanutse.Muri macrophage, gusa ΔppdK na Δmae mutants zerekanye kugabanuka kugabanuka, kandi ubushakashatsi bwakozwe na ΔppdK mutant bwemeje ko bwageze kuri niche yigana.Mu buryo nk'ubwo, onl y ΔppdK na Δmae mutants zahujwe nimbeba, iyambere yahanaguwe nicyumweru cya 10 hanyuma ikomeza kumara ibyumweru 12.Twakoze iperereza kuri cycle ya glyoxylate.Nubwo ibikorwa bya porotokoro ya aceA (isocitrate lyase) byongerewe imbaraga muburyo bukize, ihungabana rya ace nta ngaruka ryagize muri vitro cyangwa kugwiza muri macrophage cyangwa imbeba.Ibisubizo byerekana ko B. abortus ikura mu buryo budasanzwe ikoresheje isukari nke ya 6-C (na 5-C) isukari yishyurwa na glutamate kandi birashoboka ko andi acide amine yinjira muri cycle ya Krebs nta ruhare rukomeye rwa glyoxylate shunt.