Ibicuruzwa

Byakoreshejwe mugutegura CAPS buffer, zwitterionic buffer ifite akamaro murwego rwa pH 7.9-11.1.Buffer ya CAPS ikoreshwa cyane mubushakashatsi bwiburengerazuba na immunoblotting kimwe na protein ikurikirana no kumenyekana.Ikoreshwa mugukoresha amashanyarazi ya poroteyine kuri PVDF (sc-3723) cyangwa nitrocellulose (sc-3718, sc-3724).PH ndende yiyi buffer ituma iba ingirakamaro mu kohereza poroteyine hamwe na pI> 8.5.na reaction nkeya hamwe na enzymes cyangwa proteyine, ingaruka nke zumunyu.

Muri capillary zone electrophoreis umuvuduko wa electrophoreque umuvuduko wa ion ugabanuka uko kwibumbira hamwe kwinyuma ya electrolyte yumuti byiyongera.Ibi biterwa nimpinduka zijyanye na electrophoreque ya ion (muep) kimwe nimpinduka zingufu za net zibigiraho ingaruka, aribwo imbaraga zumuriro w'amashanyarazi (Eeff).Imikorere ya electrophoreque ya ion ihindurwa binyuze mumihindagurikire yubusa bwuzuye bwumuti wa electrolyte hamwe nimpinduka mubunini bwakemutse bwa ion.Eeff ihindurwa cyane cyane nimpinduka mubunini bwingaruka zidafite asimmetrie ningaruka za electrophoreque, byombi bikabuza kugenda kwa ion.Muri ubu bushakashatsi, tekinike yerekana ibimenyetso bitatu yakoreshejwe mu kwiga ingaruka ziterwa na electrolyte yibanze (0.02-0.08M 3- [cyclohexylamino] -1-propanesulfonic acide na ion (Li, Na, K, na Cs) kuri Eeff. Byagaragaye ko kwibumbira hamwe kwa electrolyte yinyuma bigira ingaruka zikomeye kuri Eeff kandi ko Eeff yegera E mugihe kwibumbira hamwe kwa electrolyte yinyuma yegera zeru. , Eeff yagabanutse. Tekinike yibimenyetso bitatu byagaragaye ko ikora neza kubyo byemezo.