Antibody ya monoclonal (mAb 8281) yihariye kuri terminal β-galactose (βGal) ya glycosphingolipide (GSL) na glycoproteine yakozwe mu mbeba zakingiwe hamwe na lipide ikomoka mu ngirabuzimafatizo nshya ya lymphocytike leukemia (BYOSE).Immuno-thin layer chromatografiya (ITLC) hamwe nipiganwa ryerekana ibipimo bitagira aho bibogamiye bya GSL, isukari yubusa, hamwe na neoglycoproteine ya sintetike yerekanaga mAb 8281 ko yakira cyane LacCer, GalCer na Gal-β-O- (CH3) 2S (CH3) 2- CONH- (Gal-β-O-CETE) ihujwe na bovine serum albumin (BSA).Isukari yanyuma nayo yagize uruhare muguhuza.Antibody ntiyigeze ikora hamwe na karubone ya hydrata hamwe na terminal αGal yubatswe hamwe nubutaka budafitanye isano.Immunoperoxidase itaziguye kandi yangiza cytometrie hamwe na mAb 8281 yerekanye irangi ryiza kumitsi myinshi, harimo imitsi yoroshye, mucosa gastrointestinal mucosa, lymph node B selile na monocytes.ITLC isesengura rya GSL igizwe na selile nshya ya B ya neoplasme ikoresheje mAb 8281 yemeje ko hariho lactosylceramide na galactosylceramide muri neoplasme yibyiciro bitandukanye byo gutandukana.Kubera umwihariko wa terminal βGal carbohydrate ibisigara, mAb 8281 irashobora kuba ingirakamaro mubisesengura ryimikorere nibikorwa bya GSL.
