Ibicuruzwa

Bumwe mu buryo bukomeye bwo kuvura indwara ya diyabete yo mu bwoko bwa 2 ni ugukoresha imiti ishobora kugabanya hyperglycaemia nyuma yo kwanduza ibuza imisemburo ya karubone.Ubu bushakashatsi bwakoze ubushakashatsi ku ngaruka ziterwa na bioassay hamwe n’ibice by’imbuto zumye pericarp ya Phaleria macrocarpa, igihingwa gakondo kirwanya diyabete, kuri α-glucosidase na α-amylase, mu rwego rwo gusobanukirwa n’uburyo bwabo bwo kurwanya diyabete, nk kimwe nigikorwa cyabo gishoboka cyo kwiyongera kwa glucose nyuma yo kwiyongera.Ibisohoka bya Methanol (ME), byabonetse mugukuramo ibisubizo bikurikiranye, igice cyacyo cyiza cyane cyamazi n-butanol agace (NBF) hamwe na flash colonment chromatographic sub-fraction (SFI), hasuzumwe kuko muri vitro α-glucosidase (umusemburo) na α-amylase (porcine) kubuza ibikorwa.Byongeye kandi, kwemeza mu bizamini bya vivo byakorewe mu mbeba ya diyabete iterwa na streptozotocine (SDRs) ikoresheje ibizamini bya glucose yo mu kanwa, sucrose na kwihanganira ibinyamisogwe. Ku bushakashatsi bwakoreshejwe cyane (1 00 μg / ml), NBF yerekanye ko yabujije α-glucosidase ( 75%) na α-amylase (87%) muri vitro (IC50 = 2,40 ± 0.23 μg / ml na 58.50 ± 0.13 μg / ml) muburyo buterwa na dose;ingaruka zagaragaye ko ziri hejuru ya 20% kurenza acarbose (55%), inhibitor ya α-glucosidase (IC50 = 3.45 ± 0.19 μg / ml).ME na SFI banabujije α-glucosidase (IC50 = 7.50 ± 0.15 μg / ml na 11.45 ± 0.28 μg / ml) na α-amylase (IC50 = 43.90 ± 0.19 μg / ml na 69.80 ± 0,25 μg / ml), ariko kugeza ku rugero ruto.Mu bushakashatsi bwa vivo hamwe nimbeba za diyabete, NBF na SFI byagabanije neza glucose yamaraso (PBG) 15.08% na 6.46%, naho agace kari munsi y’umurongo wo kwihanganira (AUC) ku kigero cya 14.23% na 12.46%, nyuma yikibazo cya sucrose yo mu kanwa (P <0.05);bityo kwemeza ibyagaragaye mubikorwa bya vitro.Izi ngaruka zo kugabanya kuri PBG na AUC zagaragaye no mu bizamini bya glucose no kwihanganira ibinyamisogwe, ariko ku rugero ruto. Ubu bushakashatsi bwerekana ko P. macrocarpa ishobora guhuza hyperglycaemia haba muri vitro ndetse no muri vivo mu guhagarika imbaraga za karubone ya hydrolysing enzymes, bigatuma igihingwa gifatika cyo gushakisha ibintu bisanzwe byo gucunga diyabete yo mu bwoko bwa 2.