Ibicuruzwa

Imipaka ya EOF iringaniza imipaka ya ITP yakoreshejwe mu gutondekanya anion ziva mu cyitegererezo cyinshi mu gihe cyo gutera inshinge za electrokinetic.Muri polystyrenesulfonate / poly (diallyldimethylammonium chloride) polyelectrolyte yometse kuri capillary, igihe umupaka wa ITP wasohokaga capillary ushobora kwongerwa muguhuza urujya n'uruza rwumupaka na EOF.Ukoresheje bis-tris-propane electrolyte, imbibi za ITP zavanywe muri capillary bitarenze 7min, mugihe mugihe ukoresheje triethanolamine umupaka wa ITP wari ukiri kuri 30% ya capillary nyuma ya 2h yatewe inshinge.Ukoresheje sisitemu, ibyiyumvo byuruvange rwa acide kama yoroshye muri 100mM Cl (-) byatejwe imbere inshuro 700-800 ukoresheje bis-tris-propane hamwe na capillary yuzuye ya sample hamwe na 5min yo guterwa electrokinetic kuri + 28kV , na 1100-1300-inshuro ukoresheje triethanolamine na 60min yo gutera inshinge za electrokinetic mubihe bimwe.Ubushobozi bwuburyo bwakoreshwa muburugero rwinshi rwerekanwe no gutondekanya capillary yuzuye yuzuyemo inkari zatewe na acide naphthalenedisulfonic, hamwe nimbibi zo gutahura zikubye inshuro 450 ugereranije n’izagerwaho hakoreshejwe inshinge zisanzwe za hydrodinamike.