Reagent nshya ya Trinder nigikomoka cyane kumazi aniline ikomoka cyane mumazi asuzumwa no gupima ibinyabuzima.Ifite ibyiza byinshi kurenza reagent ya chromogenic muburyo bwo kugena amabara ya hydrogène peroxide.Reagent nshya ya Trinder irahagaze neza kuri byombi Irashobora gukoreshwa mugukemura cyangwa muri sisitemu yo kumenya imiyoboro.Imbere ya hydrogen peroxide na peroxidase, reagent nshya ya Trinder's irashobora gukoreshwa ifatanije na 4-aminoantipyrine (4-AA) cyangwa methyl 3 Mugihe cya okiside ihuza reaction ya benzothiazole sulfonehydrazone (MBTH), irangi ryumutuku cyangwa ubururu rihamye cyane Byashizweho.Kwinjiza amarangi y'amabara afatanije na MBTH yikubye inshuro 1.5-2 ugereranije n'irangi rifatanije na 4-AA;icyakora, 4-AA Igisubizo kirahamye kuruta igisubizo cya MBTH.Substrate ihindurwamo okiside na okiside kugirango itange hydrogen peroxide.Ubwinshi bwiyi hydrogen peroxide ihuye nubunini bwa substrate.Kubwibyo, ingano ya substrate irashobora kugenwa niterambere ryamabara ya okiside ihuza reaction.Glucose, inzoga, acyl-CoA, na cholesterol birashobora gukoreshwa mugutahura izo substrate hamwe na reagent ya Trinder na 4-AA.Hano hari reagent 10 nshya ya Trinder.Muri reagent nshya ya Trinder, TOOS niyo ikoreshwa cyane.Nyamara, kuri substrate yihariye, kugerageza ubwoko butandukanye bwibisobanuro bya Trinder birakenewe kugirango utezimbere uburyo bwiza bwo kumenya.
