Ibicuruzwa

Sigma reseptor 1 (σR1) ni poroteyine itari opioid transembrane ishobora gukora nka chaperone ya molekuline kuri endoplasmic reticulum-mitochondrial membrane.Ligands ya σR1, nka (+) - pentazocine [(+) - PTZ], itanga ibimenyetso byerekana neuroprotection retinal muri vivo no muri vitro.Muminsi ishize twasesenguye retine phenotype yimbeba zidafite σR1 (σR1 KO) hanyuma tureba morphologie isanzwe yimikorere nimikorere yimbeba zikiri nto (ibyumweru 5-30) ariko twagabanije ibisubizo bibi bya scotopic (nSTRs), gutakaza ingirabuzimafatizo (RGC), no guhungabana ya optic nerv axons ijyanye nimikorere yimbere yumwaka 1.Aya makuru yatumye tugerageza hypothesis ko σR1 ishobora kuba ingenzi mukurinda amashyamba adakira;diyabete yakoreshejwe nk'icyitegererezo cyo guhangayika karande.Kumenya niba σR1 isabwa kuri (+) - Ingaruka za neuroprotective ya PTZ, RGC y'ibanze yitandukanije n'ubwoko bw'ishyamba (WT) na σR1 KO imbeba zahuye na okiside ya xanthine-xanthine (10 µM: 2 mU / ml) gutera imbaraga za okiside ihari cyangwa idahari (+) - PTZ.Urupfu rwakagari rwasuzumwe na terminal deoxynucleotidyl transfert dUTP nick end labels (TUNEL) isesengura.Kugira ngo hamenyekane ingaruka ziterwa na chronique ku mikorere ya RGC, diyabete yatewe mu byumweru 3 C57BL / 6 (WT) na σR1 KO imbeba, ikoresheje streptozotocine itanga amatsinda ane: WT nondiabete (WT non-DB), diabete ya WT (WT-DB ), σR1 KO itari DB, na σR1 KO-DB.Nyuma yibyumweru 12 bya diyabete, mugihe imbeba zari zifite ibyumweru 15, umuvuduko wamaraso (IOP) waranditswe, hakorwa ibizamini bya electrophysiologique (harimo no kumenya nSTRs), kandi umubare wa RGC wabaruwe mubice byamateka ya retina.Mu bushakashatsi bwa vitro bwerekanye ko .Mu bushakashatsi bwimyitwarire idakira iterwa na diyabete, IOP yapimwe mumatsinda ane yimbeba yari murwego rusanzwe;icyakora, habayeho kwiyongera gukomeye muri IOP yimbeba σR1 KO-DB (16 ± 0.5 mmH g) ugereranije nandi matsinda yapimwe (σR1 KO non-DB, WT non-DB, WT-DB: ~ 12 ± 0,6 mmHg ).Kubijyanye no gupima electrophysiologique, nSTRs ya σR1 KO imbeba zitari DB zasa nimbeba WT itari DB mubyumweru 15;icyakora, bari hasi cyane muri σR1 KO-DB imbeba (5 ± 1 µV) ugereranije nandi matsinda, harimo, cyane cyane, σR1 KO-nonDB (12 ± 2 µV).Nkuko byari byitezwe, umubare wa RGCs muri σR1 KO imbeba zitari DB wasaga nimbeba WT itari DB mu byumweru 15, ariko kubera ibibazo bidakira bya diyabete habaye RGC nkeya muri retina yimbeba σR1 KO-DB.Iyi niyo raporo yambere kwerekana bidasubirwaho ko ingaruka za neuroprotective za (+) - PTZ zisaba σR1.σR1 KO imbeba yerekana imiterere isanzwe ya retina n'imikorere kumyaka mike;icyakora, iyo uhuye nibibazo bidakira bya diyabete, habaho kwihuta kwimikorere yimikorere ya retina mumbeba ya σR1 KO kuburyo imikorere mibi ya ganglion igaragara mugihe cyambere cyane kuruta imbeba za nondiabete σR1 KO.Amakuru ashyigikira hypothesis ivuga ko σR1 igira uruhare runini muri modu itinda guhagarika umutima kandi ishobora kuba intego yingenzi yindwara zifata umwijima.