X-GAL CAS: 7240-90-6 98% Ifu yera-yera ya Crystalline
| Umubare wa Cataloge | XD90008 |
| izina RY'IGICURUZWA | X-Gal (5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) |
| URUBANZA | 7240-90-6 |
| Inzira ya molekulari | C14H15BrClNO6 |
| Uburemere bwa molekile | 408.63 |
| Ibisobanuro birambuye | -2 kugeza kuri 6 ° C. |
| Amategeko agenga ibiciro | 29400000 |
Kugaragaza ibicuruzwa
| Kugaragara k'umuti | Sobanura neza, ibara ritagira ibara ryumuhondo (50mg / ml muri DMF: MeOH, 1: 1) |
| Guhinduranya neza | -61.5 +/- 1 |
| Kugaragara | Ifu yera-yera-ifu ya kristaline |
| HPLC | min 99% |
| Gukemura (5% muri DMF) | Gukemura (5% w / v, DMF) |
| Amazi KF | max 1% |
| Suzuma (HPLC kuri Anhydrous Basis) | min 98% w / w |
Imikoreshereze ya X-gal
X.Uru ruganda rwashizwemo na Jerome Horwitz nabafatanyabikorwa mu 1964. Izina ryimiti ryemewe akenshi rigabanywa kugirango ridasobanutse neza ariko kandi ninteruro zitoroshye nka galaktoside ya bromochloroindoxyl.X kuva indoxyl irashobora kuba isoko ya X mukugabanuka kwa X-gal.X-gal ikoreshwa kenshi mubinyabuzima bya molekuline kugirango isuzume ko hari enzyme, β-galactosidase, mu mwanya wabyo isanzwe, β-galactoside.Irakoreshwa kandi mukumenya ibikorwa byiyi misemburo muri histochemie na bacteriology.X-gal ni imwe muri indoxyl glycoside na esters zitanga ibara ry'ubururu budashonga busa n'irangi rya indigo bitewe na hydrolysis ya enzyme.
X.X. -indigo - 2, ibicuruzwa byubururu cyane bidashobora gukemuka.X.Ibi kandi bituma bagiteri β-galactosidase (bita lacZ) ikoreshwa nkumunyamakuru mubikorwa bitandukanye.
Mu isesengura ryibice bibiri, gal-galactosidase irashobora gukoreshwa nkumunyamakuru kugirango umenye poroteyine zikorana.Muri ubu buryo, amasomero ya genome arashobora kugenzurwa kugirango imikoranire ya poroteyine ikoresheje umusemburo cyangwa sisitemu ya bagiteri.Iyo hari imikoranire myiza hagati ya poroteyine zerekanwa, bizavamo guhuza indangarubuga ya enterineti.Niba porotokoro ihujwe na lacZ gene, umusaruro wa gal-galactosidase, bivamo gushiraho ubukoroni-pigmented coloni imbere ya X-gal, bizerekana rero imikoranire myiza hagati ya poroteyine.Ubu buhanga bushobora kugarukira gusa mu kwerekana amasomero yubunini buri munsi ya 106. Gutsindira neza X-gal nabyo bitera impumuro mbi igaragara kubera guhindagurika kwa indole.
Nka X-gal ubwayo idafite ibara, kuba hari ibara ryubururu rishobora gukoreshwa nkikizamini cyo kuba hariho β-galactosidase ikora.
Kumenyekanisha byoroshye enzyme ikora ituma gene ya βgalactosidase (gene lacZ) ikoreshwa nkumunyamakuru gene mubikorwa bitandukanye.
