Ibicuruzwa

Senseri ya PH yahimbwe na anodic electrodepositing ya iridium oxyde (AEIROFs) kuri microelectrode kuri chip hanyuma igashyirwaho poly (Ethyleneimine) (PEI) kugirango itekane neza.Ibi byerekana super-Nernstian igisubizo kuri pH kuva pH 4.0 kugeza 7.7 muri buffer ya chloride idafite fosifate.Ubuso bwa chip bwashizweho na fibronectine kugirango hafatwe ingirabuzimafatizo ya Aortic endothelial selile (PAECs).Ubushobozi bwakazi bwa pH sensor yo gukurikirana impinduka zikomeye zaho pH zakozweho ubushakashatsi mukubyutsa PAEC hamwe na trombine.Ibisubizo byacu byerekana ko trombine yateje acide acide idasanzwe ya PAECs no gusesa fibronectine, bigatuma pH yaho igabanuka.Ikoreshwa rya PD98059, inhibitor ya mitogen ikora protein kinase (MAPK) inhibitor, yagabanije aside aside idasanzwe kandi kwiyongera kwa pH byaho byagaragaye.Ubu bushakashatsi bwerekana ko ibyuma bya pH byacu bishobora koroshya iperereza ryibisubizo bikaze byimikorere ya selile mugukurikirana igihe nyacyo, pH ihinduka rya selile zifatanije na sensor.