Ibicuruzwa

MicrosRNA yunvikana cyane (miRNA) biosensor ikoresha ruthenium oxyde nanoparticle (RuO (2) NP) -yatangije polymerisiyasi ya 3,3'-dimethoxybenzidine (DB) hamwe na miRNA yerekana urugero rwa poly insuline (3,3'-dimethoxybenzidine) (PDB) film isobanurwa muriki gikorwa.Biosensor yakozwe na monolayeri ivanze ya oligonucleotide yo gufata (CPs) na 4-mercaptoaniline kuri electrode ya zahabu.Nyuma yo kuvanga hamwe na RuO (2) NP yashizweho intego ya miRNA, imvange ya DB / H (2) O (2) muri pH 5.0 0.10 M ya acetate yakoreshejwe kuri biosensor.NPs ya RuO (2) ikora nka polymerisation itangiza / itanga umusemburo wa polymerisation ya DB.Kandi imvange ya anionic miRNA imirongo hamwe na CP yubusa ikora nkicyitegererezo, ikayobora ububiko bwa PDB.Ingano ya PDB yabitswe hamwe nimbaraga zayo zo gukingira bifitanye isano itaziguye no kwibanda ku ntego ya miRNA mu gisubizo.Ibizamini bya electrochemical impedance spectroscopic byerekanaga ko umubano wumurongo-woherejwe-kurwanya-kwibanda kuri 6 .0 fM kugeza kuri 2.0 pM byagezweho nyuma yiminota 60 ya incububasi ivanze DB / H (2) O (2).Nta guhuza kwambukiranya hagati ya miRNA yabanjirije na miRNA ikuze na bike cyane bivangavanga hagati yumuryango wa miRNA bifitanye isano rya bugufi ndetse no murwego rumwe-rudahuye.Iyi biosensor ishingiye kuri impedance itanga ubundi buryo bushimishije bwo kwerekana imvugo ya miRNA kandi irashobora gutuma habaho iterambere ryimikorere ya miRNA yerekana imiterere.