Ibicuruzwa

Itandukaniro rya fibroblast ya orbital muri adipocytes ikuze hamwe no kwegeranya kwa tipusi ya adipose byagaragaye mugutezimbere kwa orbitopathie ya Graves (GO).Autophagy igira uruhare muri adipogenezi, ariko bike birazwi kubyerekeye uruhare rwa autophagy mugutangiza no gutera imbere kwa GO.Intego yubu bushakashatsi ni ugukora ubushakashatsi ku ruhare rwa autophagy mu gutera indwara ya GO.Orbital adipose / ihuza ingirabuzimafatizo zivuye ku barwayi bafite GO ndetse no mu masomo asanzwe, ndetse na fibroblast ya orbital yonyine.Adipogenezi yatewe no gukoresha uburyo butandukanye bwo gutandukanya hydrogène cyangwa idafite hydrogène, kandi autophagy yakoreshwaga hakoreshejwe bafilomycine A1 na Atg5 igenewe imisatsi migufi RNA (shRNA).Autophagosomes yamenyekanye na microscopi ya electron.Kugaragaza genes zifitanye isano na autofagy hamwe na adipogenezi zijyanye no kwandukura ibintu byasesenguwe nigihe nyacyo cyo guhindura transcription-polymerase urunigi rwerekana hamwe na / cyangwa isesengura ryiburengerazuba.Gukusanya ibitonyanga bya Lipid byari exami ned byamavuta Red Red O irangi.Icyuka cya autofagique cyari kinini muri selile GO kuruta muri selile zitari GO (p <0.05).Kugaragaza genes zifitanye isano na autofagy byari hejuru cyane mubice bya GO na selile kuruta muri bagenzi babo batari GO.Interleukin-1β yiyongereye LC3-II, p62, na Atg7 proteyine muri selile GO.Ikwirakwizwa rya Autophagosome ryerekanwe kumunsi wa 4 wa adipogenezi kandi ryaragabanutse kumunsi wa 10, hamwe na lipide itonyanga.Kugaragaza poroteyine za LC3 na p62 byiyongereye mu masaha 48 yo gutandukana kandi byagabanutse buhoro buhoro kuva ku munsi wa 4 kugeza ku wa 10. Kuvura Bafilomycine A1 na Atg5 gukomanga na shRNA byabujije kwirundanya kwa lipide no guhagarika ibimenyetso bya adipogenic.Autophagy yariyongereye muri tissue ya GO na selile ugereranije utugingo ngengabuzima na GO, byerekana ko autophagy igira uruhare muri GO pathogenez.Autophagy manipulation irashobora kuba intego yo kuvura GO.