Ibicuruzwa

Nubwo fluor izwiho gutera amagufwa, uburyo bwibanze ntabwo bwumvikana neza.Ubushakashatsi buherutse gukorwa bwerekanye inzira ya Wnt / β-catenin nkinzira ikomeye yerekana kasake muri biologiya yamagufwa.Ubushakashatsi bwacu bwambere bwerekanye uruhare rushoboka rwinzira ya Wnt inzira yo gukora amagufwa yimbeba zidakira za fluoride, ariko uburyo ntiburasobanuka.Ubushakashatsi buriho bwerekanye uruhare rwa Wnt / β-catenin yerekana ibimenyetso bya fluoride iterwa na osteoblastique.Twifashishije imbeba y'ibanze ya osteoblasts, twerekanye ko fluoride yazamuye cyane ikwirakwizwa rya osteoblasts hamwe na fosifate ya alkaline fosifate (ALP) kimwe na mRNA imvugo yerekana ibimenyetso bitandukanya amagufwa, harimo ubwoko bwa I kolagen (COL1A1), ALP na osteonectine.Twasanze kandi fluor yatewe na fosifora kuri serine 473 ya Akt na serine 9 ya glycogen synthase kinase-3β (GSK3β), ibyo bikaba byaratumye GSK-3β ibuzwa hanyuma ikwirakwizwa rya kirimbuzi β-catenine, nkuko bigaragazwa na blot yo muburengerazuba hamwe nisesengura rya immunofluorescence .Byongeye kandi, fluor nayo yatumye imvugo ya Wnt-igenewe gene runt ijyanye no kwandukura ibintu 2 (Runx2).Icyangombwa, ingaruka nziza ya fluor kubikorwa bya ALP hamwe na mRNA imvugo ya COL1A1, ALP, osteonection na Runx2 yavanyweho na DKK-1, uhagarika reseptor ya Wnt / β-catenin.Ufatiye hamwe, ubushakashatsi bwerekana ko fluor itera itandukaniro rya osteoblastique binyuze muri Akt- na GSK-3β biterwa na enterineti ya Wnt / β-catenin yerekana inzira muri osteoblasts.Ibyavuye mu bushakashatsi bitanga ubumenyi bushya muburyo bwo gukora fluoride muri osteoblastogenez.