Ibicuruzwa

LecA (PA-IL) ni inyigisho ya cytotoxic na adhesine ikorwa na Pseudomonas aeruginosa ihuza galactoside hydrophobique ifite umwihariko kandi ifitanye isano.Dukoresheje lecA-egfp ibisobanuro byo guhuza hamwe na immunoblot isesengura rya materix ya biofilm idasanzwe, twerekana ko lecA igaragarira mu ngirabuzimafatizo zikuze za biofilm.Muri biofilm ihagaze neza kuri polystirene ndetse nicyuma kitagira umwanda, ubujyakuzimu bwa biofilm hamwe nuburinganire bwubutaka byagabanutse kubera ihinduka rya lecA kandi byongerewe imbaraga muri LecA itanga umusaruro PAO-P47.Ubuso bwa biofilm buterwa nababyeyi, PAO-P47 ariko ntabwo ihinduka rya lecA kuri coupons yicyuma naryo ryabujijwe gukura imbere ya isopropyl-beta-D-thiogalactoside (IPTG) cyangwa p-nitrophenyl-alpha-D-galactoside ( NPG).Byongeye kandi, ibinyabuzima byo mu bwoko bwa biofilm bikuze byakozwe mugihe hatabayeho galaktoside ya hydrophobique irashobora gutatanya hiyongereyeho IPTG.Ibinyuranye, kongeramo p-nitrophenyl-alpha-L-fucose (NPF) ifitanye isano rya bugufi n’inyigisho za P. aeruginosa LecB (PA-IIL) n ntacyo byagize ku miterere ya biofilm cyangwa gutatana.Iterambere rya planktonique ya P. aeruginosa PAO1 ntiryigeze rihungabanywa no kuba hari IPTG, NPG cyangwa NPF, nta nubwo imbaraga zashoboye gukoresha ayo masukari nk'isoko ya karubone, byerekana ko ingaruka zagaragaye ku miterere ya biofilm zatewe no guhagarika amarushanwa ya LecA-ligand guhuza.Ibisubizo nkibi byabonetse no kuri biofilm ikura mubihe bigenda byoroha kuri coupons, byerekana ko LecA igira uruhare mubwubatsi bwa biofilm ya P. aeruginosa mubihe bidukikije bitandukanye.

Alpha-L-fucosidase (EC 3.2.1.51) ibasha kurekura ibisigazwa bya t-fucosyl kumurongo wuruhande rwa xyloglucan oligosaccharide byagaragaye mumababi yibiti bya Arabidopsis.Byongeye kandi, alpha-L-fucosidase ifite umwihariko wa substrate wasukuwe mumababi ya cabage (Brassica oleracea) kugirango itange umurongo umwe kuri SDS-PAGE.Inzira ebyiri za peptide zabonetse muri iri tsinda rya poroteyine, kandi zarakoreshejwe mu kumenya gene ya Arabidopsis code ya alpha-fucosidase dusaba guhamagara AtFXG1.Byongeye kandi, habonetse gene ya Arabidopsis hamwe na homologiya hamwe na alpha-L-fucosidase izwi, kandi twasabye kuyita AtFUC1.Byombi AtFXG1 na ATFUC1 byagaragaye mu buryo butandukanye mu ngirabuzimafatizo za Pichia pastoris ndetse n'ibikorwa bya alpha-L-fucosidase byashyizwe mu muco.Alpha-L-fucosidase yashizweho na AtFXG1 yakoraga kurwanya oligosaccharide kuva xyloglucan XXFG kimwe no kurwanya 2'-fucosyl-lactitol ariko ntabwo yarwanyaga p-nitrophenyl-alpha-L-fucopyranoside.Ariko, AtFUC1 yerekanwe muburyo butandukanye yakoraga gusa kurwanya 2'-fucosyl-lactitol.Rero, ibyambere bigomba kuba bifitanye isano na xyloglucan metabolism.