Ibicuruzwa

Muburyo bwa kinetic angiotensin-ihindura enzyme (ACE), buffer ifatika kandi nziza ni 80 mmol / L ya borate kuri pH 8.2 (37 dogere C).Icyiciro cyakererewe kigaragara mubitekerezo, kandi 5-min incubation ya substrate na plasma birasabwa mbere yo gupima kinetic.Substrate, N-Uburyo buteganijwe bukoresha igipimo cya 1: 9 plasma-kuri-reagent.Urutonde rwumurongo rugera kuri 170 U / L, rugereranya hydrolysis ya 25%.Kwinjira kwa FAPGG kugenwa no gupima itandukaniro ryo kwinjiza hagati ya 1.0 mmol / L FAPGG nibisubizo byibicuruzwa.Ubudahemuka bwumuraba bugenzurwa no kumenya agaciro kateganijwe kwinjizwa nigisubizo cya FAPGG, naho gutandukana kwa 1.0-nm kuva 345 nm bihindura iyinjira rya 15.5%.Ubusobanuro bwa ACE bwerekana hafi 60 na 100 U / L ni 3.5% na 2,4% mugice kimwe na 2.9% na 2.6% hagati yicyiciro.Intera yerekana (2,5 kugeza kuri 97.5 ku ijana) ni 41-139 U / L, kandi nta tandukaniro riri hagati yagaciro kubagabo nabagore.