Ibicuruzwa

Carbonic anhydrase ikora: ubushakashatsi bwambere bwibikorwa byububiko bwumuntu isoform VI hamwe na aside amine na amine.

Ibanga isozyme ya anhydrase ya karubone yumuntu (hCA, EC 4.2.1.1), hCA VI, yarakoronijwe, iragaragazwa, kandi yezwa muri sisitemu yo kwerekana bagiteri.Ibipimo bya kinetic ya reaction ya CO (2) byagaragaye ko hCA VI ifite ak (injangwe) ya 3,4 x 10 (5) s (-1) na k (injangwe) / K (M) ya 4.9 x 10 (7) M (-1) s (-1) (kuri pH 7.5 na dogere 20 C).hCA VI ifite ibikorwa byingenzi bya catalitiki kubikorwa bya physiologique, yuburyo bumwe bwubunini nka isoform iboneka hose CA I cyangwa transembrane, ibibyimba bifitanye isano na isozyme CA IX.Urukurikirane rwa aside amine na amine byerekanwe gukora nkibikorwa bya CA VI, hamwe nibikorwa bihinduka.l-Ibye, l-Trp, na dopamine byagaragaje intege nke za CA VI zikora (K (A) s ziri hagati ya microM 21-42), naho d-His, d-Phe, l-DOPA, l-Trp, serotonine , na pyridyl-alkylamine zimwe zari zikora neza, hamwe na K (A) s murwego rwa 13-19 microM.Abakoresha neza CA VI ni l-Phe, d-DOPA, l-Tyr, 4-amino-l-Phe, na histamine, hamwe na K (A) s iri hagati ya microM 1.23-9.31.Aba activateur bose bazamura k (injangwe), nta ngaruka bigira kuri K (M), bitabira bityo igipimo kigena intambwe mukuzunguruka kwa catalitiki, reaction ya proton hagati ya enzyme ikorera hamwe nibidukikije.