Ibicuruzwa

Guhindura ibinyabuzima bya methyl-β-D-glucopyranoside kugeza kumurongo muremure alkyl glucoside ihujwe ningirabuzimafatizo β-glucosidase ya Pichia etchellsii.

Muri ubu bushakashatsi, twakoze ubushakashatsi ku mikoreshereze ya selile zose za Pichia etchellsii muguhuza urunigi rurerure rwa alkyl glucoside.Methyl-β-d-glucopyranoside (MG) yakoreshejwe mugusubiza hamwe na alcool zirimo ibinure, n-hexanol, n-octanol, n-decanol na n-dodecanol muguhuza glucoside ya alkyl.Uburyo bwambere bwo kubyitwaramo bwabanje kunozwa muburyo bwa miriyoni 2,5 kugirango habeho synthesis ya octyl glucoside (OG) kandi byari 8% byamazi, 100mM MG na 6h yigihe cyo kubyitwaramo kandi byatanze umusaruro wa 53%.Umubare ntarengwa wa transglucosylation / hydrolysis ya 2,79 wabonetse kuri 100mM MG itanga umusaruro mwinshi.Ukurikije uko ibintu bimeze neza, reaction yakoreshwaga kurwego rwa ml 50 bigatuma ≈ 60% ihinduka MG kuri OG.Uburyo bworoshye bwo gukora ibintu byamazi ya chromatografiya yateguwe kugirango habeho ingano ya glucoside yo mu rwego rwo hejuru ikoresheje indangururamajwi.Umubare ntarengwa wa 27% na 13% wabonetse kuri decyl-, na dodecyl-β-d-glucopyranoside;