Ibicuruzwa

Kimwe nizindi virusi nziza za RNA, tombusvirus yigana na virusi igana virusi (VRC).VRC igizwe na virusi ya p92 yanditswe na RNA iterwa na RNA polymerase (RdRp), virusi ya p33 RNA chaperone, hamwe na poroteyine nyinshi zakira hamwe.Kugirango ube RdRp ikora nyuma yubusobanuro bwayo, poroteyine yo kwigana p92 igomba kwinjizwa muri VRC, hanyuma igakurikizwa.Twerekanye mbere mumasemburo adafite selile-ashingiye ku bushakashatsi bwerekana ko gukora virusi ya Tomato bushy stunt (TBSV) RdRp bisaba ibintu byakiriye neza.Muri iki kiganiro, turerekana poroteyine yubushyuhe bwa selile 70 (Hsp70) nkibintu bifatanyabikorwa byakiriwe kugirango ibikorwa bya N-byaciwe na recombinant TBSV RdRp.Byongeye kandi, molekile ntoya ishingiye kumikorere ya Hsp70 ibuza synthesis ya RNA na tombusvirus RdRp muri vitro.Byongeye kandi, twerekana ko fosifolipide idafite aho ibogamiye, aribyo fosifatiidylethanolamine (PE) na fosifatidylcholine (PC), byongera ibikorwa bya RdRp muri vitro.Ibinyuranye, phosphatidylglycerol (PG) yerekana ingaruka zikomeye kandi ziganje mubikorwa bya vitro RdRp.Turerekana kandi ko PE na PC bitera RdRp-virusi hiyongereyeho RNA [(+) RNA] imikoranire, mugihe PG ibuza guhuza RNA virusi na RdRp.Dushingiye ku gukangura no kubuza uruhare rwa fosifolipide zitandukanye mu bikorwa bya tombusvirus RdRp, turasaba ko lipide igizwe na membrane subcellular membrane ishobora gukoreshwa na tombusvirus kugirango igenzure inteko nshya ya VRC mugihe cyo kwandura.Icyorezo cya RNA gishingiye kuri RNA polymerase. .Izi ngamba zishobora kwirinda virusi ya RNA ikwirakwizwa muri cytosol yakwihutira kwinjiza kwinjiza RNA iterwa na selile antiviral ibisubizo.Mugihe cyo guterana kwa membrane-bigana kwigana, virusi RdRp itangira gukora binyuze muburyo butumvikana neza butuma RdRp ishoboye synthesis.Ukoresheje TBSV RdRp, twerekana ko hamwe na selire Hsp70 chaperone hamwe na fosifolipide idafite aho ibogamiye byorohereza RdRp gukora muri vitro.Ibinyuranye, phosphatidylglycerol (PG) igira ingaruka zikomeye zo guhagarika ibikorwa bya vitro RdRp no gukorana kwa RdRp-virusi ya RNA, byerekana ko microdomain ya membrane ikikije RdRp igira ingaruka zikomeye kubushobozi bwayo bwo guhuza RNA.Rero, gukora virusi RdRp birashoboka biterwa nibice byinshi byakiriye muri selile zanduye.