Ibicuruzwa

Icyari kigamijwe muri ubu bushakashatsi kwari ukuranga imiterere y’irondakoko no gusuzuma umutekano wa 129 Enterococcus yonyine ikomoka kuri vino ikorerwa fermentation ya malolactique.Ibiranga genetike byakozwe na polymorphic ADN-PCR byateganijwe byerekanwa genotypes 23.25 yitaruye uhagarariye genotypes zose zamenyekanye nka Enterococcus faecium na PCR yihariye y’ubwoko kandi isuzumwa kugira ngo irwanye antibiyotike, kuba hari virusi ya virusi ndetse n’ubushobozi bwa aminobiogenic, haba muri decarboxylase na divayi.Ubushobozi bwa aminobiogenic muri vino bwasesenguwe imbere (assay 1) no kudahari (assay 2) ya Oenococcus oeni CECT 7621. Kurwanya tetracycline, cotrimoxazol, vancomycin na teicoplanine byagaragajwe na 96% byimiterere, ariko ntanumwe muribo wigeze akora ubushakashatsi. virusi.Ubwoko bwose bwarimo gene ya tyrosine decarboxylase (tdc), mugihe 44% byari byiza kuri tyramine muburyo bwa decarboxylase.Ibice bitanu kuri 25 byonyine byarokotse muri vino nyuma yiminsi irindwi yubushakashatsi, kandi mugihe hamenyekanye ibipimo bya amine biogenic muri divayi byagenwe na HPLC, gusa izo divayi zabayemo amoko atanu yarokotse yarimo amine ya biyogenike.Histamine, putrescine na cadaverine byagaragaye muri divayi zivuye mu bushakashatsi bwombi, nubwo ubushakashatsi bwibanze ku bushakashatsi bwa 2. Tyramine na fenylethylamine byagaragaye gusa O. oeni adahari.Ubu bushakashatsi bugira uruhare mu bumenyi bwumutekano wa enterococci ijyanye no gukora divayi.