Ibicuruzwa

Gusaza kwa chronologique ya selile yimisemburo ikoreshwa nkicyitegererezo cyo gusaza kwingirabuzimafatizo ya muntu nyuma ya mitoto.Umusemburo Saccharomyces cerevisiae ukura kuri glucose imbere ya sulfate ya amonium ikoreshwa cyane mubushakashatsi bwo gusaza.Twasesenguye gusaza kwigihe cyumusemburo Hansenula polymorpha ukura mubihe bisaba metabolisme yambere ya peroxisome kugirango ikure.Ubuzima bwigihe cyigihe cya H. polymorpha bwiyongera cyane mugihe ingirabuzimafatizo zikuze kuri methanol cyangwa Ethanol, zikoreshwa na enzymes za peroxisome, ugereranije no gukura kuri glucose ibyo ntibisaba peroxisomes.Iminsi mike ya H. polymorpha kuri glucose ahanini iterwa na aside irike, mugihe bishoboka cyane ko ROS idafite uruhare runini.Gukura kwingirabuzimafatizo kuri methanol / methylamine aho kuba methanol / sulfate ya ammonium byatumye ubuzima bwiyongera.Ibi ntaho byari bihuriye na acide yo hagati.Twerekana ko okiside ya methylamine na peroxisomal amine oxydease ya carbone mubihe byinzara ya carbone isubiza ible kugirango umuntu yongere igihe.Igicuruzwa cya methylamine oxydeire fordehide irushijeho kuba okiside bigatuma NADH itanga, igira uruhare mukwiyongera kwa ATP no kugabanya urwego rwa ROS mugice gihagaze. Twanzuye ko metabolism yibanze ya peroxisome yazamuye ubuzima bwigihe cya H. polymorpha.Byongeye kandi, amahirwe yo kubyara NADH mubihe byinzara ya karubone ikomoka kuri azote kama ifasha gukomeza kwagura ubuzima bwakagari.Kubwibyo, gusobanura isesengura rya CLS mumusemburo bigomba kubamo ingaruka zishoboka kumbaraga zingirabuzimafatizo.