Ibicuruzwa

Kimwe mu bintu bibuza gukoresha imiti ivura indwara zandurira mu ndwara ni isoko ikwiye ya selile.Chondrocytes, ubwoko bumwe gusa bwa selile ya karitsiye, ikura muri vitro mubihe byumuco kugirango yagure umubare wimikorere itakaza fenotipike hamwe nubushobozi bwo kubyara ingirabuzimafatizo.Muri ubu bushakashatsi tumenye ko sisitemu yumuco ya serumu- no gukura-idafite-ibice bitatu (3D) bigarura ubushobozi bwa chondrocytes yaciwe yo gukora tissue ya karitsiye muri vitro, inzira irimo sox9.Bondine articular chondrocytes yanyujijwe kabiri kugeza Emera kwagura umubare w'utugari (P2) kandi utere imbere cyane kuri 3D collagen-ubwoko-II-bushyizweho na membrane mu bitangazamakuru byinshi bya glucose byuzuyemo insuline na dexamethasone (SF3D).Ingirabuzimafatizo zaranzwe nyuma yo kwaguka kwa monolayeri no gukurikiza umuco wa 3D ukoresheje cytometrike, imvugo ya gene, hamwe namateka.Impinduka zambere mukumenyesha inzira ya transduction mugihe cya redifferentiat ion yaranzwe.Selile P2 yerekanaga progenitor imeze nka antigen umwirondoro wa 99% CD44 (+) na 40% CD105 (+) hamwe numwirondoro wa gene yerekana ingirabuzimafatizo za interzone.P2 muri SF3D yerekanye genes ya chondrogeneque hamwe na matrix idasanzwe.Kugabanya insuline ya insuline (IR) hamwe na HNMPA- (AM3) cyangwa inzira ya PI-3 / AKT kinase (ikoreshwa no kuvura insuline) hamwe na Wortmannin yabujije synthesis ya kolagen.HNMPA- (AM3) yagabanije imvugo ya gen2 ya Col2, Col11, na IR kimwe na Sox6 na -9.Co-immunoprecipitation hamwe na chromatin immunoprecipitation isesengura rya selile HNMPA- (AM3) -yakozwe na selile yerekanaga guhuza coactivators Sox6 na Med12 hamwe na Sox9 ariko byagabanije Sox9-Col2a1 guhuza.Turasobanura uburyo bwumuco mushya utuma umubare wa chondrocytes wiyongera. hyaline isa na karitsiye ya tissue igice kimwe na insuline-yunganiwe na Sox9-Col2a1 guhuza.Ubushobozi bwimyenda yabyaye hakoreshejwe ubu buryo bwo gukoreshwa mugusana hamwe bigomba gusuzumwa muri vivo.