Glucose-6-Fosifate Dehydrogenase CAS: 9001-40-5
| Umubare wa Cataloge | XD90375 |
| izina RY'IGICURUZWA | Glucose-6-Fosifate Dehydrogenase |
| URUBANZA | 9001-40-5 |
| Inzira ya molekulari | N / A. |
| Uburemere bwa molekile | N / A. |
| Ibisobanuro birambuye | 2 kugeza 8 ° C. |
| Amategeko agenga ibiciro | 35079090 |
Kugaragaza ibicuruzwa
| NAD u / mgP | 90590 NAD ibice kuri proteine ya mg |
| u / ml | Raporo agaciro. |
| mgP / ml | .5 7.5 |
| % PHI | ≤0.02% |
| Suzuma | 99% |
| % 6-PGDH | ≤0.003% |
| Kugaragara | Ifu yera |
| % CK | ≤0.002% |
| %AK | ≤0.002% |
Isesengura rya proteome-yagutse ya fosifora yibintu biracyari ikibazo gikomeye cyo gusesengura kubera urusobe runini rwa poroteyine ya fosifora.Muri iki gikorwa, turasuzuma ubwuzuzanye bwa Lys-N, Lys-C, na trypsin bijyanye nubushobozi bwabo bwo gutanga umusanzu mu isesengura ryisi yose ya fosifore.Uburyo bunonosoye bwo guhanahana amakuru-pH akomeye yakoreshejwe mugutandukanya neza N-kurangiza acetylated, fosifora, na peptide idahinduwe.Hafi ya 5036 ya fosiforeptide idakabije irashobora kumenyekana hamwe nigipimo cyo kuvumbura ibinyoma cya <1% kuva mg 1 ya proteine ukoresheje guhuza imisemburo itatu.Amakuru yacu yerekanaga ko guhuzagurika hagati yamakuru ya fosifopteptide yakozwe na protease zitandukanye byari marginal, mugihe guhuzagurika hagati yamakuru abiri asa nayandi makuru yabonetse yasanze byibuze inshuro 4 hejuru.Muri ubu buryo, gukoresha parallel ya Lys-N na trypsin byatumye ubwiyongere bwa 72% bwumubare wa fosiforeptide wamenyekanye ugereranije na d na trypsin wenyine, mugihe ubushakashatsi bwa trypsin bwigana gusa bwiyongereyeho 25%.Rero, mugihe twibanze gusa kuri data ya trypsin na Lys-N, twasanze 4671 fosifopeptide idakabije.Isesengura ryakozwe ku mbuga zagaragaye ryerekanye ko amakuru ya Lys-N na trypsin yatunganijwe mu buryo butandukanye cyane bwa fosifora, bikomeza kwerekana ko uburyo bwa Multrotease bufite agaciro kanini mu isesengura rya fosifore.
