Ibicuruzwa

Twateje imbere udushya duto two kubangamira RNA (siRNA) dukoresheje urwego rwa ternary hamwe na polyethyleneimine (PEI) na acid-polyglutamic aside (γ-PGA), byerekanaga ingaruka zo guceceka kandi nta cytotoxicity.Binary complexes ya siRNA hamwe na PEI yari hafi 73-102 nm mubunini buke na 45-52 mV muri ζ-bishoboka.Ingaruka zo gucecekesha ibigo bya siRNA / PEI byiyongereye hamwe no kwiyongera kwa PEI, kandi ibigo bya siRNA / PEI bifite igipimo cyamafaranga arenga 16 byerekanaga luciferase gukomanga kumurongo wimbeba ya kanseri ya kanseri ya kanseri buri gihe yerekana luciferase (selile26 / Luc selile).Nyamara, cytotoxicity ikomeye hamwe na agglutination yamaraso byagaragaye murwego rwa siRNA / Lipofectamine hamwe na siRNA / PEI16.Kwishyuza cationic complexes hamwe na anionic compound byavuzwe ko aribwo buryo butanga ikizere cyo gutsinda ubwo burozi.Twateguye rero ternary complexes ya siRNA hamwe na PEI (igipimo cyamafaranga 16) hiyongereyeho γ-PGA kugirango tugabanye cytotoxicity no gutanga siRNA.Nkuko byari byitezwe, cytotoxicity ya ternary complexs yagabanutse hamwe no kwiyongera kwa γ-PGA, byagabanije ζ -bishobora kuba byikigo.Ingaruka ikomeye yo guceceka ugereranije na siRNA / Lipofectamine yavumbuwe mubigo bitatu birimo γ-PGA hamwe nubushakashatsi bwa anionic.Kwinjiza kwinshi kwa ternary muri selile26 / Luc byemejwe na microcopi ya fluorescence.Tumaze kugera ku gukomeretsa gene yahinduwe mu buryo budasanzwe, hasuzumwe ubushobozi bw’ikigo cyo guhuza no gukomeretsa gene yo mu rugo ya endogenous, glyceraldehyde 3-fosifate dehydrogenase (GAPDH), yasuzumwe mu tugari twa B16-F10.Igice cya ternary (siRNA / PEI16 / γ-PGA12 complex) cyerekanye ingaruka zikomeye za GAPDH.Rero, twateje imbere sisitemu yo gutanga siRNA.