Ibicuruzwa

Ingaruka zitunguranye z'ibyiciro bimwe na bimwe bya anticoagulants yabaye osteoporose ishobora kuba byibuze igice, ifitanye isano n'imikorere ya mesenchymal stem selile (MSC).Icyari kigamijwe muri ubu bushakashatsi kwari ukugereranya ingaruka za fondaparinux (FDP), antithrombotic yanditswe na gakondo gakondo ikoreshwa cyane na heparin, tinzaparin (TZP) ku ikwirakwizwa rya MSC no gutandukana.MSCs yitandukanije n'amagufa ya trabecular y’abarwayi 14 b’ihungabana n’uburyo bwo gusya bushingiye kuri kolagenase kandi bwagutse mu bihe bisanzwe kugeza ku gice cya 3. Gukwirakwiza no gutandukanya MSCs na chondrocytes na osteoblasts byasuzumwe cyangwa hatabariwemo FDP na TZP hakoreshejwe bisanzwe muri vitro. asuzuma hamwe ningeri nyinshi yibiyobyabwenge.Flow cytometrie yakoreshejwe kuri MSC phenotyping.Mumyaka yize mumatsinda (17-74 ans) inshuro ya MSC mubice byarekuwe na kolagenase yari selile 641/10 (6) (intera 110-2,158) kandi imiterere yabakuze yari days4 iminsi / abaturage bakikuba kabiri.Imico yari ifite phenotype isanzwe ya MSC (CD73 +, CD105 +, CD146 +, CD106 +, na CD166 +).Ikwirakwizwa ry'utugari ryasuzumwe byombi bigize fibroblast (CFU-F) hamwe na tetrazolium umunyu XTT.Muri ubwo buryo bwombi, ikwirakwizwa rya MSC ryabujijwe no kongerwaho TZP, cyane cyane ku kwibanda cyane.Ibinyuranye, FDP nta ngaruka yagize ku ikwirakwizwa rya MSC.Itandukaniro rya Osteogenic no gutandukanya chondrogeneque ntabwo byatewe no kongeramo TZP cyangwa FDP.Mu gihe ikwirakwizwa rya MSC, ariko ridatandukanye, ryatewe nabi na TZP, nta kimenyetso cyerekana ingaruka mbi za FDP muri iyi sisitemu ya vitro yerekana ko ikoreshwa neza mu mikorere y'amagufwa.