Ibicuruzwa

Dioxydejene ya Tet 5-methylcytosine itera ADN demethylation ikora 5-hydroxymethylcytosine nibindi bicuruzwa bya okiside.Tet1 na Tet2 bigaragarira cyane muri selile pluripotent selile kandi bikagabanywa kuburyo butandukanye muri selile somatike, ariko uburyo bwo kwandukura ntibisobanutse.Hano twasobanuye porotokoreri niyongera muri domaine muri Tet1 na Tet2.Muri 15-kb "superenhancer" ya Tet1, hariho imbuga ebyiri zo gutangira kwandukura (TSSs) hamwe nuburyo butandukanye bwo gukora mugihe cyiterambere.Agace ka 6-kb gatera imbere hejuru ya TSS ya kure ikora cyane muri selile na pluripotent selile, yigenga itangaza imvugo ya Tet1 muri sisitemu ya transgenji, kandi ihita ihura na methylation ya ADN no guceceka nyuma yo gutandukanya selile zifite umuco na epiblast kavukire.Isegonda ya kabiri ya TSS kumanuka, ifitanye isano na CpG ikungahaye cyane ya porotokoro ikungahaye, ikorwa nuwiyongera mubaturanyi mumyanya mito ya embryonic stem selile (ESCs) hamwe na epiblast isa na selile (EpiLCs).Tet2 ifite ikirwa cya CpG p romoter hamwe nibikorwa byigenga bya pluripotency hamwe na ESC yihariye intera ya interagenic yongera;ibya nyuma bigabanywa vuba muri EpiLCs.Ubushakashatsi bwacu bugaragaza uburyo butandukanye bwo gutondekanya inyandiko kuri Tet1 na Tet2 mugihe leta ihinduranya iterambere ryambere.Abanyamakuru bashya ba transgenji bakoresheje Tet1 na Tet2 cis-igenga domaine barashobora gufasha gutandukanya impinduka zidafite ishingiro muri leta zifite imbaraga nyinshi hamwe nubwoko butandukanye bwa epigenetike.