Ibicuruzwa

Igikorwa cyibinyabuzima: BCIP (umunyu wa BCIP p-toluidine; umunyu wa X-fosifate p-toluidine) ni chromogenic substrate yo gutahura amabara yerekana ibikorwa bya fosifata ya alkaline.

Uburozi bwa Shiga (Stxs) bukorwa na enterohemorrhagic Escherichia coli (EHEC), itera kwandura abantu hamwe ningaruka zica.Imirongo ngengabuzima ya Vero, ikomoka ku mpyiko zo muri Afurika icyatsi kibisi, zerekana urugero rwa zahabu mu kumenya ingaruka za cytotoxique ya Stxs.Nubwo bakoresha isi yose, ubumenyi bujyanye nuburyo nyabwo bwa reseptor ya Stx reseptor glycosphingolipide (GSLs) no guterana kwabo muri lipide ni bibi.Hano turerekana isesengura ryuzuye ryimiterere ya Stx reseptor GSLs no kuyikwirakwiza kuri membrane idashobora kwangirika (DRMs), byateguwe kuva selile Vero-B4 kandi bigakoreshwa nkibingana na lipide.Twabonye globotriaosylceramide (Gb3Cer) na globotetraosylceramide (Gb4Cer) nk'ibikoresho bya GSL byakira Stx1a, Stx2a, na Stx2e dukoresheje TLC yuzuye hamwe na MS.Reseptor ya Stx idasanzwe, globopentaosylceramide (Gb5Cer, Galβ3GalNAcβ3Galα4Galβ4Glcβ1Cer), yari izwi cyane (usibye Gb3Cer na Gb4Cer) na Stx2e, yaranzwe muburyo bwuzuye.Lipoforms ya reseptor ya Stx GSLs wasangaga ahanini ibika moeram ceramide igizwe na sphingosine (d18: 1) na C24: 0 / C24: 1 cyangwa C16: 0 aside irike.Byongeye kandi, gufatanya na marike ya lipide, SM na cholesterol, muri DRMs byasabye GSL gufatanya na microdomain ya membrane.Ubu bushakashatsi butanga urufatiro rwo kurushaho gushakisha ingaruka ziterwa na lipide raft ifitanye isano na Stx reseptors yo gukomeretsa uburozi bwatewe na selile Vero-B4.