Ibicuruzwa

APS-5 ni insimburangingo ya chemiluminescent ishingiye kuri 9,10-dihydroacridine, ikoreshwa cyane cyane muri ELISA gutahura ibice hamwe na fosifatike ya alkaline fosifata AP;9,10-dihydroacridine muburyo bwa APS-5 Imiterere ya hydroacridine irashobora gusohora urumuri ubudahwema, butajegajega kandi neza mugikorwa cya AP, kandi APS-5 irakwiriye cyane kubikorwa bya fosifata yibisubizo hamwe na fosifata enzyme ifitanye isano na immunoassay.

Nka probe ya luminescent, ikoreshwa mukwiga chip ya gene.Igisubizo cyanditseho acridan (9,10-dihydroacridine) nka substrate na fosifata ya alkaline, itanga chemiluminescence ikabije.Itanga ibyiyumvo byikirenga kandi byoroshye gukoresha ikoreshwa rya alkaline fosifata mugihe cyo gutahura chemiluminescent.Fosifata ya alkaline iboneka mugihe kitarenze 10-19 mol, impinga byihuse kugirango igabanye igihe cyo gutahura no kongera ibicuruzwa, kandi umusozi wumurongo wa kalibrasi uteganijwe hamwe na logarithmic ingana na 1.0.Ingano imwe cyangwa nyinshi ya enzyme itanga ingano imwe cyangwa myinshi ya luminescence, luminescence ikomeza - ntibisaba cyane mugihe cyo gusuzuma.Ubukomezi bwa Luminescence burashobora gusomwa uhereye kumurongo wa kalibrasi yakozwe mugihe icyo aricyo cyose, kandi ibisubizo byisesengura ntabwo byumva ubushyuhe buri hagati ya 22 ° C - 35 ° C, bikagabanya ukuri gukenewe kugirango ubushyuhe bugabanuke.

akarusho:

1 Ubukangurambaga bukabije - ibimenyetso bya poroteyine munsi ya 5pg birashobora kumenyekana;

2 Imbaraga nyinshi cyane - impinga yumucyo irashobora kugerwaho mugihe gito;

3 Luminescence ikomeza kandi ihamye - inzira yayo ya luminescence ntabwo ihindurwa nubushyuhe buri muri 25-35 and, kandi ntibisaba kugenzura ubushyuhe.

Kugereranya ikoreshwa rya luminescent isanzwe ikoreshwa APS-5 (193884-53-6) na AMPPD (122341-56-4)

Byombi APS-5 (193884-53-6) na AMPPD (122341-56-4) ni insimburangingo ya chemiluminescent ya fosifata ya alkaline, kandi byombi byitwa ultra-high sensitivite detection reagents, ubwo rero reagent zombi ziri mubushakashatsi Nibyiza kandi byiza Ikibi ni ikihe?Nigute ushobora guhitamo mugihe uyikoresha?

Ku ruhande rumwe, iyo APS-5 ikoreshwa nka substrate ya alkaline fosifata, ibyiyumvo byo gutahura bishobora kugera kuri 10-19 yibibabi, igihe cyo gukura amabara gishobora kugera kumpera ntarengwa mumasegonda 10, kandi fluorescence ikamara igihe kirekire igihe.Muri urwo rwego, APS 5 irashobora kuvugwa ko ifite ibintu byiza cyane.Nyamara, APS-5 igarukira mubushyuhe bwo gusaba, kandi ibara ryayo ryerekana ubushyuhe nibyiza munsi ya 20'C.Ku rundi ruhande, iyo AMPPD ikoreshwa nka substrate yo gutahura fosifata ya alkaline, sensitivite yo gutahura nayo yitwa ultra-high sensitivite, ariko ntishobora kugera ku rwego rwa APS 5. Igihe cyo gukura kw'amabara kigera ku mpinga ntarengwa ku masegonda 30 , na fluorescence igihe kirekire.AMPPD ni nziza cyane mumiterere yavuzwe haruguru, ariko mubyukuri irutwa na APS5, ariko ibara ryayo ryerekana ubushyuhe ntabwo rikomeye nka APS5, kandi ubushyuhe bwo kubika bwa 2--8C bworoshye kuruta APS-5.Muri make, nubwo APS-5 irenze AMPPD muburyo bwo kumenya neza, itakaza mumutekano.Uburyo bwo guhitamo biterwa nibikenewe byihariye byo gutahura.

1. Intangiriro ya APS-5 (193884-53-6):

Izina ry'icyongereza: APS-5

Numero ya CAS: 193884-53-6

Inzira ya molekulari: C21H15ClNO4PS Na2

Uburemere bwa molekuline: 489.819

Kugaragara: ifu yera-yera

Ibirimo: ≥98%

Ibisobanuro: 1g, 5g, 10g

Imiterere yo kubika: -20 ℃ kubungabunga

(4-Chlorophenylmercapto).Itanga ibyiyumvo byikirenga kandi byoroshye gukoresha ikoreshwa rya alkaline fosifata mugihe cyo gutahura chemiluminescent.Igisubizo cyanditseho acridan (9,10-dihydroacridine) nka substrate na fosifata ya alkaline, itanga chemiluminescence ikabije.APS-5 ni reagent nziza yo kumenya ibikorwa bya fosifata hakoreshejwe ibisubizo hamwe na fosifata enzyme ifitanye isano na immunoassays.Nka luminescent substrate, APS-5 ifite sensibilité yo hejuru hamwe nigihe gito cyo kubyitwaramo.

2. Intangiriro irambuye ya AMPPD (122341-56-4)

Izina ry'icyongereza: AMPPD

URUBANZA No: 122341-56-4

Inzira ya molekulari: C18H23O7P

Uburemere bwa molekile: 382.344781

Kugaragara: ifu yera

Isuku: ≥97%

Ibisobanuro: 1g, 5g, 10g

Imiterere yo kubika: -20 ℃ kubungabunga

Icyitonderwa: Igomba kuba yumye kugirango wirinde gushonga kenshi na lyophilisation